Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Synechococcus elongatus |
Crystallization (Comment) | Organism |
---|---|
in complex with isoniazid, sitting drop vapor diffusion method, using 4.3 M sodium formate and 100 mM sodium citrate, pH 6.4 | Synechococcus elongatus |
Protein Variants | Comment | Organism |
---|---|---|
S308T | the mutant shows strongly reduced activity compared to the wild type enzyme | Synechococcus elongatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
isoniazid | wild type enzyme, at pH 7.8 and 37°C | Synechococcus elongatus | |
10.18 | - |
isoniazid | mutant enzyme S308T, at pH 7.8 and 37°C | Synechococcus elongatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Synechococcus elongatus | - |
O2 + H2O | - |
? | |
2 H2O2 | Synechococcus elongatus PCC7942 | - |
O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus | Q31MN3 | - |
- |
Synechococcus elongatus PCC7942 | Q31MN3 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography and Mono Q column chromatography | Synechococcus elongatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Synechococcus elongatus | O2 + H2O | - |
? | |
2 H2O2 | - |
Synechococcus elongatus PCC7942 | O2 + H2O | - |
? | |
isoniazid + H2O2 | - |
Synechococcus elongatus | oxidized isoniazid + H2O | - |
? | |
isoniazid + H2O2 | - |
Synechococcus elongatus PCC7942 | oxidized isoniazid + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Synechococcus elongatus |