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Literature summary for 1.11.1.21 extracted from

  • Kudalkar, S.N.; Campbell, R.A.; Li, Y.; Varnado, C.L.; Prescott, C.; Goodwin, D.C.
    Enhancing the peroxidatic activity of KatG by deletion mutagenesis (2012), J. Inorg. Biochem., 116, 106-115.
    View publication on PubMed
Search for more literature for 1.11.1.21

Protein Variants

Protein Variants Comment Organism
DELTA200-214 mutation eliminates catalase activity, but variant is substantially more active as peroxidase Escherichia coli
DELTA209-228 variant is substantially more active as peroxidase Escherichia coli
DELTAL193-N228 mutant lacking Large Loop1, mutation eliminates catalase activity, but variant is substantially more active as peroxidase Escherichia coli
Y226F mutation eliminates catalase activity, but variant is substantially more active as peroxidase Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant Y22F, pH 5.0, 23°C Escherichia coli
0.086
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTA200-214, pH 5.0, 23°C Escherichia coli
0.31
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild-type, pH 5.0, 23°C Escherichia coli
1.06
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTAL193-N228, pH 5.0, 23°C Escherichia coli
1.2
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTA209-228, pH 5.0, 23°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
 Escherichia coli O2 + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli ? + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50.2
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTA200-214, pH 5.0, 23°C Escherichia coli
83
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild-type, pH 5.0, 23°C Escherichia coli
97
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant Y22F, pH 5.0, 23°C Escherichia coli
740
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTAL193-N228, pH 5.0, 23°C Escherichia coli
1100
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTA209-228, pH 5.0, 23°C Escherichia coli
9600
-
 H2O2 mutant DELTA200-214, catalase activity, pH 7.0, 23°C Escherichia coli
11000
-
 H2O2 wild-type, catalase activity, pH 7.0, 23°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
270
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild-type, pH 5.0, 23°C Escherichia coli
580
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTA200-214, pH 5.0, 23°C Escherichia coli
750
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTAL193-N228, pH 5.0, 23°C Escherichia coli
960
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant DELTA209-228, pH 5.0, 23°C Escherichia coli
2300
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant Y22F, pH 5.0, 23°C Escherichia coli