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Literature summary for 1.11.1.21 extracted from

  • Cendrin, F.; Jouve, H.M.; Gaillard, J.; Thibault, P.; Zaccai, G.
    Purification and properties of a halophilic catalase-peroxidase from Haloarcula marismortui (1994), Biochim. Biophys. Acta, 1209, 1-9.
    View publication on PubMed
Search for more literature for 1.11.1.21

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate additions of ammonium sulfate even at weak concentrations (10 to 70 mM) stimulate catalatic activity measured in the presence of 1.5 M NaC1 by about 30% Haloarcula marismortui
NaCl with NaC1, maximum activity is observed near 0.5-0.7 M Haloarcula marismortui

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition of the catalase activity of hCP is seen with 3-amino-l,2,4-triazole Haloarcula marismortui
NaCl the peroxidatic activity on diaminobenzidine shows a continuous decrease with increasing NaCl concentration Haloarcula marismortui

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 H2O2 peroxidatic activity, pH 6.0, 25°C Haloarcula marismortui
2.5
-
 H2O2 catalatic activity, pH 7.5, 25°C Haloarcula marismortui

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81290
-
 mass spectrometry Haloarcula marismortui
94000
-
 SDS-PAGE Haloarcula marismortui

Organism

Organism UniProt Comment Textmining
Haloarcula marismortui
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose column chromatography, DEAE cellulose column chromatography, dialysis, hydroxyapatite column chromatography, and ACA 44 gel filtration Haloarcula marismortui

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
 crude extract, catalatic activity, at 20°C and pH 7.5, in the presence of 1.5 M NaC1 Haloarcula marismortui
851
-
 after 170fold purification, catalatic activity, at 20°C and pH 7.5, in the presence of 1.5 M NaC1 Haloarcula marismortui

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Haloarcula marismortui oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
diaminobenzidine + H2O2
-
 Haloarcula marismortui oxidized diaminobenzidine + H2O
-
 ?
guaiacol + H2O2
-
 Haloarcula marismortui tetraguaiacol + H2O
-
 ?
H2O2
-
 Haloarcula marismortui O2 + H2O
-
 ?
o-dianisidine + H2O2
-
 Haloarcula marismortui oxidized o-dianisidine + H2O
-
 ?
pyrogallol + H2O2
-
 Haloarcula marismortui ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
HCP
-
 Haloarcula marismortui

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 peroxidatic activity in the absence of NaCl, with diaminobenzidine as electron donor Haloarcula marismortui
7.5
-
 catalatic activity in molar concentrations of NaCl, with diaminobenzidine as electron donor Haloarcula marismortui

Cofactor

Cofactor Comment Organism Structure
heme
-
 Haloarcula marismortui