Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
H2O2 | purified enzyme is sensitive to H2O2 concentrations higher than 10 mM | Synechococcus elongatus PCC 7942 = FACHB-805 | |
additional information | the enzyme is insensitive to the eukaryotic catalase inhibitor 3-amino-1,2,4-triazole | Synechococcus elongatus PCC 7942 = FACHB-805 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
H2O2 | catalase activity, in 67 mM phosphate buffer (pH 7.0), at 25°C | Synechococcus elongatus PCC 7942 = FACHB-805 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80500 | - |
2 * 80500, native PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus PCC 7942 = FACHB-805 | - |
formerly Synechococcus sp. strain PCC 6301 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Fractogel EMD 650(s) DEAE column chromatography, phenyl-Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S300 gel filtration | Synechococcus elongatus PCC 7942 = FACHB-805 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.4 | - |
crude extract, in 67 mM phosphate buffer (pH 7.0), at 25°C | Synechococcus elongatus PCC 7942 = FACHB-805 |
785 | - |
after 178fold purification, in 67 mM phosphate buffer (pH 7.0), at 25°C | Synechococcus elongatus PCC 7942 = FACHB-805 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | O2 + H2O | - |
? | |
additional information | the enzyme does not accept electrons from ascorbate, glutathione, and NADH | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | - |
? | |
o-dianisidine + H2O2 | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | oxidized o-dianisidine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 80500, native PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7200 | - |
H2O2 | catalase activity, in 67 mM phosphate buffer (pH 7.0), at 25°C | Synechococcus elongatus PCC 7942 = FACHB-805 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 75-80% of maximum initial rates are still retained at pH 6.0 and 9.0 | Synechococcus elongatus PCC 7942 = FACHB-805 |
6.5 | 7.5 | catalase and peroxidase activities | Synechococcus elongatus PCC 7942 = FACHB-805 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0272 | - |
cyanide | in 67 mM phosphate buffer (pH 7.0), at 25°C | Synechococcus elongatus PCC 7942 = FACHB-805 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Synechococcus elongatus PCC 7942 = FACHB-805 | isoelectric focusing | - |
4.7 |