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Literature summary for 1.11.1.21 extracted from

  • Zamocky, M.; Furtmueller, P.G.; Bellei, M.; Battistuzzi, G.; Stadlmann, J.; Vlasits, J.; Obinger, C.
    Intracellular catalase/peroxidase from the phytopathogenic rice blast fungus Magnaporthe grisea: expression analysis and biochemical characterization of the recombinant protein (2009), Biochem. J., 418, 443-451.
    View publication on PubMed
Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 DE3 Star cells Pyricularia grisea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8
-
 H2O2 at pH 6.0 and 25°C Pyricularia grisea

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pyricularia grisea
-
-
intracellular
-
 Pyricularia grisea 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83170
-
 theoretical molar mass of monomeric KatG1 with one heme b and a His6-tag Pyricularia grisea
88000
-
 4 * 88000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea
90000
-
 2 * 90000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea
160000
-
 dimeric KatG1, SDS-PAGE Pyricularia grisea
180000
-
 homodimer, gel filtration Pyricularia grisea
352000
-
 homotetramer, gel filtration Pyricularia grisea

Organism

Organism UniProt Comment Textmining
Pyricularia grisea A4QUT2 strain MA 829
-
Pyricularia grisea A4R5S9 strain MA 829
-
Pyricularia grisea ATCC MYA-4617 A4QUT2 strain MA 829
-
Pyricularia grisea ATCC MYA-4617 A4R5S9 strain MA 829
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, Superdex 200 gel filtration Pyricularia grisea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pyricularia grisea ?
-
 ?
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?
ethanol + H2O2
-
 Pyricularia grisea acetaldehyde + ?
-
 ?
ethanol + H2O2
-
 Pyricularia grisea ATCC MYA-4617 acetaldehyde + ?
-
 ?
guaiacol + H2O2
-
 Pyricularia grisea ?
-
 ?
guaiacol + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?
o-dianisidine + H2O2
-
 Pyricularia grisea ?
-
 ?
o-dianisidine + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?
pyrogallol + H2O2
-
 Pyricularia grisea ?
-
 ?
pyrogallol + H2O2
-
 Pyricularia grisea ATCC MYA-4617 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 90000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea
homotetramer 4 * 88000, about 95% of the protein exists in homodimeric form, gel filtration Pyricularia grisea

Synonyms

Synonyms Comment Organism
catalase/peroxidase
-
 Pyricularia grisea
KatG
-
 Pyricularia grisea
KatG1 located intracellularly Pyricularia grisea
KatG2 located extracellularly Pyricularia grisea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 65 95% loss of catalase activity after 22 h at 40°C, 95% loss of catalase activity after 330 min at 45°C, 95% loss of catalase activity after 48 min at 50°C, 95% loss of catalase activity after 15 min at 55°C, 95% loss of catalase activity after 8 min at 60°C, 95% loss of catalase activity after 1 min at 65°C Pyricularia grisea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7010
-
 H2O2 at pH 6.0 and 25°C Pyricularia grisea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 also acts as a versatile peroxidase with a pH optimum in the range 5.0-5.5, using both one-electron [2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), o-dianisidine, pyrogallol, or guaiacol] and two-electron (Br-, I- or ethanol) donors Pyricularia grisea
6
-
-
 Pyricularia grisea

Cofactor

Cofactor Comment Organism Structure
heme
-
 Pyricularia grisea
heme oxidoreductase with a predominant five-coordinated high-spin heme b, ferric MagKatG1 exhibits the typical bands of a heme b-containing peroxidase Pyricularia grisea

pI Value

Organism Comment pI Value Maximum pI Value
Pyricularia grisea isoelectric focusing
-
 5.7
Pyricularia grisea calculated from amino acid sequence
-
 6