Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21 DE3 pLysS cells | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | in 50 mM acetate buffer, pH 5.0, at 23°C | Escherichia coli | |
27 | - |
H2O2 | in 100 mM phosphate buffer, pH 7.0, at 23°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and phenyl-Sepharose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | KatP has stronger catalase than peroxidase activity | Escherichia coli | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
H2O2 | KatP has stronger catalase than peroxidase activity | Escherichia coli | O2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatP | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
77 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | in 50 mM acetate buffer, pH 5.0, at 23°C | Escherichia coli | |
18000 | - |
H2O2 | in 100 mM phosphate buffer, pH 7.0, at 23°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
maximal peroxidase activity | Escherichia coli |
7.2 | - |
maximal catalase activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | ferric KatP possesses a mixture of pentacoordinate and hexacoordinate high-spin heme iron | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | periplasmic catalase-peroxidases contributes to bacterial virulence | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | in 50 mM acetate buffer, pH 5.0, at 23°C | Escherichia coli | |
640 | - |
H2O2 | in 100 mM phosphate buffer, pH 7.0, at 23°C | Escherichia coli |