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show all sequences of 1.11.1.20

Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily

Moriuchi, H.; Koda, N.; Okuda-Ashitaka, E.; Daiyasu, H.; Ogasawara, K.; Toh, H.; Ito, S.; Woodward, D.F.; Watanabe, K.; J. Biol. Chem. 283, 792-801 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
(NH4)2SO4
stimulates
Sus scrofa
ammonium sulfate
the activity of the purified recombinant enzyme is stimulated about 4fold by 1.5 M ammonium sulfate
Mus musculus
Cloned(Commentary)
Commentary
Organism
construction of an expression plasmid using polyhistidine-tagged peptide-fused vector and mouse cDNA encoding a 201-amino acid polypeptide with molecular weight of 21669 Da and expression the protein in Escherichia coli; expressed in Escherichia coli BL21(DE3) cells
Mus musculus
Engineering
Amino acid exchange
Commentary
Organism
C44S
below 1% of wild-type activity; the mutant is almost inactive with less than 1% of wild type activity
Mus musculus
C44S/C47S
below 1% of wild-type activity; the mutant is almost inactive with less than 1% of wild type activity
Mus musculus
C47S
mutant shows reduced activity compared to the wild type enzyme; retains 63% of wild-type activity
Mus musculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
cumene hydroperoxide
competitive inhibition of prostamide F synthase activity
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
thioredoxin
pH 7.0, 24°C
Mus musculus
0.0069
-
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
pH 7.0, 24°C, i.e. prostaglandin H2
Mus musculus
0.0069
-
H2O2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.0069
-
prostaglandin H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.007
-
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
i.e. prostaglandin H2, pH 7.0, 24°C
Sus scrofa
0.0076
-
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
pH 7.0, 24°C, i.e. prostamide H2
Mus musculus
0.0076
-
prostamide H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.014
-
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
i.e. prostamide H2, pH 7.0, 24°C
Sus scrofa
0.026
-
cumene hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C; pH 7.0, 24°C
Mus musculus
0.4
-
t-butyl hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.4
-
tert-butyl hydroperoxide
pH 7.0, 24°C
Mus musculus
1.6
-
H2O2
pH 7.0, 24°C
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mus musculus
5829
-
endoplasmic reticulum
-
Mus musculus
5783
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
(NH4)2SO4
1.5 M, stimulates about 4fold
Mus musculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
SDS-PAGE
Mus musculus
20000
-
x * 20000, SDS-PAGE
Sus scrofa
21670
-
calculated from amino acid sequence
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
Q9DB60
-
-
Sus scrofa
A9CQL8
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
; Ni2+-agarose column chromatography and Q Sepharose column chromatography
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
brain
activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs; highest activity in the spinal cord and brain
Mus musculus
-
brain
-
Sus scrofa
-
cecum
lowest activity in the cecum
Mus musculus
-
epididymis
-
Mus musculus
-
heart
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
kidney
-
Mus musculus
-
large intestine
-
Mus musculus
-
liver
-
Mus musculus
-
lung
-
Mus musculus
-
ovary
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
spinal cord
highest activity in the spinal cord and brain; localized in the superficial layer of the dorsal horn, in motor neurons of the ventral horn, and in glia of the white matter of the spinal cord. Activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
stomach
-
Mus musculus
-
testis
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
thymus
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
uterus
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
vas deferens
-
Mus musculus
-
vesicular gland
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.053
-
prostamine F synthase activity, pH 7.0, 24°C
Sus scrofa
0.062
-
prostaglandin F synthase activity, pH 7.0, 24°C
Sus scrofa
0.25
-
prostamine F synthase activity, pH 7.0, 24°C; substrate: tert-butyl hydroperoxide, pH 7.0, 24°C
Mus musculus
0.28
-
substrate: cumene hydroperoxide, pH 7.0, 24°C; substrate: H2O2, pH 7.0, 24°C
Mus musculus
0.69
-
prostaglandin F synthase activity, pH 7.0, 24°C
Mus musculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
prostaglandin D2 and prostaglandin E2 are not reduced
687797
Mus musculus
?
-
-
-
-
additional information
neither prostaglandin E2 nor prostamide D2 serve as a substrate even at the concentration of 1.5 mM
687797
Mus musculus
?
-
-
-
-
additional information
the recombinant enzyme requires thioredoxin as a reducing equivalent donor more efficiently than NADPH, NADH, or GSH
687797
Mus musculus
?
-
-
-
-
thioredoxin + (5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide i.e. prostamide H2
687797
Sus scrofa
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide i.e. prostamide F2alpha
-
-
?
thioredoxin + (5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide i.e. prostamide H2. Prostaglandin H2 and prostamide H2 are the best substrates
687797
Mus musculus
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide i.e. prostamide F2alpha
-
-
?
thioredoxin + (5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate i.e. grostaglandin H2
687797
Sus scrofa
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate i.e. prostaglandin F2alpha
-
-
?
thioredoxin + (5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate i.e. prostaglandin H2. Prostaglandin H2 and prostamide H2 are the best substrates. Cys44 is essential for the catalytic activity of prostamide/PGF synthase
687797
Mus musculus
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate i.e. prostaglandin F2alpha
-
-
?
thioredoxin + cumene hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + H2O + 2-phenylpropan-2-ol
-
-
-
?
thioredoxin + cumene hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + ?
-
-
-
?
thioredoxin + H2O2
-
687797
Mus musculus
thioredoxin disulfide + 2 H2O
-
-
-
?
thioredoxin + H2O2
-
687797
Mus musculus
thioredoxin disulfide + ?
-
-
-
?
thioredoxin + prostaglandin H2
prostaglandin H2 is one of the best substrates
687797
Mus musculus
thioredoxin disulfide + prostaglandin F2alpha
-
-
-
?
thioredoxin + prostamide H2
prostamide H2 is one of the best substrates
687797
Mus musculus
thioredoxin disulfide + prostamide F2alpha
-
-
-
?
thioredoxin + t-butyl hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + ?
-
-
-
?
thioredoxin + tert-butyl hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + H2O + tert-butanol
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 21670, stimulation by ammonium sulfate suggests that the enzyme may be a dimer or oligomer instead of a monomer, calculated from sequence
Mus musculus
?
x * 20000, SDS-PAGE
Sus scrofa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
24
-
assay at
Mus musculus
24
-
assay at
Sus scrofa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.001
-
thioredoxin
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.09
-
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
pH 7.0, 24°C, i.e. prostamide H2
Mus musculus
0.09
-
prostamide H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.09
-
t-butyl hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.09
-
tert-butyl hydroperoxide
pH 7.0, 24°C
Mus musculus
0.1
-
cumene hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C; pH 7.0, 24°C
Mus musculus
0.1
-
H2O2
in 0.1 M KPB (pH 7.0), at 24°C; pH 7.0, 24°C
Mus musculus
0.25
-
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
pH 7.0, 24°C, i.e. prostaglandin H2
Mus musculus
0.25
-
prostaglandin H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
; optimal pH for the prostamide F synthase activity
Mus musculus
7
-
assay at
Sus scrofa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
thioredoxin
-
Mus musculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
(NH4)2SO4
stimulates
Sus scrofa
ammonium sulfate
the activity of the purified recombinant enzyme is stimulated about 4fold by 1.5 M ammonium sulfate
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
construction of an expression plasmid using polyhistidine-tagged peptide-fused vector and mouse cDNA encoding a 201-amino acid polypeptide with molecular weight of 21669 Da and expression the protein in Escherichia coli; expressed in Escherichia coli BL21(DE3) cells
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
thioredoxin
-
Mus musculus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C44S
below 1% of wild-type activity; the mutant is almost inactive with less than 1% of wild type activity
Mus musculus
C44S/C47S
below 1% of wild-type activity; the mutant is almost inactive with less than 1% of wild type activity
Mus musculus
C47S
mutant shows reduced activity compared to the wild type enzyme; retains 63% of wild-type activity
Mus musculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cumene hydroperoxide
competitive inhibition of prostamide F synthase activity
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
thioredoxin
pH 7.0, 24°C
Mus musculus
0.0069
-
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
pH 7.0, 24°C, i.e. prostaglandin H2
Mus musculus
0.0069
-
H2O2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.0069
-
prostaglandin H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.007
-
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
i.e. prostaglandin H2, pH 7.0, 24°C
Sus scrofa
0.0076
-
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
pH 7.0, 24°C, i.e. prostamide H2
Mus musculus
0.0076
-
prostamide H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.014
-
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
i.e. prostamide H2, pH 7.0, 24°C
Sus scrofa
0.026
-
cumene hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C; pH 7.0, 24°C
Mus musculus
0.4
-
t-butyl hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.4
-
tert-butyl hydroperoxide
pH 7.0, 24°C
Mus musculus
1.6
-
H2O2
pH 7.0, 24°C
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mus musculus
5829
-
endoplasmic reticulum
-
Mus musculus
5783
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
(NH4)2SO4
1.5 M, stimulates about 4fold
Mus musculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
SDS-PAGE
Mus musculus
20000
-
x * 20000, SDS-PAGE
Sus scrofa
21670
-
calculated from amino acid sequence
Mus musculus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
; Ni2+-agarose column chromatography and Q Sepharose column chromatography
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
brain
activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs; highest activity in the spinal cord and brain
Mus musculus
-
brain
-
Sus scrofa
-
cecum
lowest activity in the cecum
Mus musculus
-
epididymis
-
Mus musculus
-
heart
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
kidney
-
Mus musculus
-
large intestine
-
Mus musculus
-
liver
-
Mus musculus
-
lung
-
Mus musculus
-
ovary
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
spinal cord
highest activity in the spinal cord and brain; localized in the superficial layer of the dorsal horn, in motor neurons of the ventral horn, and in glia of the white matter of the spinal cord. Activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
stomach
-
Mus musculus
-
testis
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
thymus
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
uterus
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
vas deferens
-
Mus musculus
-
vesicular gland
; activity is the highest in spinal cord and brain followed by thymus, adrenal gland, heart, and genital organs
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.053
-
prostamine F synthase activity, pH 7.0, 24°C
Sus scrofa
0.062
-
prostaglandin F synthase activity, pH 7.0, 24°C
Sus scrofa
0.25
-
prostamine F synthase activity, pH 7.0, 24°C; substrate: tert-butyl hydroperoxide, pH 7.0, 24°C
Mus musculus
0.28
-
substrate: cumene hydroperoxide, pH 7.0, 24°C; substrate: H2O2, pH 7.0, 24°C
Mus musculus
0.69
-
prostaglandin F synthase activity, pH 7.0, 24°C
Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
prostaglandin D2 and prostaglandin E2 are not reduced
687797
Mus musculus
?
-
-
-
-
additional information
neither prostaglandin E2 nor prostamide D2 serve as a substrate even at the concentration of 1.5 mM
687797
Mus musculus
?
-
-
-
-
additional information
the recombinant enzyme requires thioredoxin as a reducing equivalent donor more efficiently than NADPH, NADH, or GSH
687797
Mus musculus
?
-
-
-
-
thioredoxin + (5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide i.e. prostamide H2
687797
Sus scrofa
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide i.e. prostamide F2alpha
-
-
?
thioredoxin + (5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide i.e. prostamide H2. Prostaglandin H2 and prostamide H2 are the best substrates
687797
Mus musculus
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxy-N-(2-hydroxyethyl)prosta-5,13-dien-1-amide i.e. prostamide F2alpha
-
-
?
thioredoxin + (5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate i.e. grostaglandin H2
687797
Sus scrofa
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate i.e. prostaglandin F2alpha
-
-
?
thioredoxin + (5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate i.e. prostaglandin H2. Prostaglandin H2 and prostamide H2 are the best substrates. Cys44 is essential for the catalytic activity of prostamide/PGF synthase
687797
Mus musculus
thioredoxin disulfide + (5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate
(5Z,9alpha,11alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dienoate i.e. prostaglandin F2alpha
-
-
?
thioredoxin + cumene hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + H2O + 2-phenylpropan-2-ol
-
-
-
?
thioredoxin + cumene hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + ?
-
-
-
?
thioredoxin + H2O2
-
687797
Mus musculus
thioredoxin disulfide + 2 H2O
-
-
-
?
thioredoxin + H2O2
-
687797
Mus musculus
thioredoxin disulfide + ?
-
-
-
?
thioredoxin + prostaglandin H2
prostaglandin H2 is one of the best substrates
687797
Mus musculus
thioredoxin disulfide + prostaglandin F2alpha
-
-
-
?
thioredoxin + prostamide H2
prostamide H2 is one of the best substrates
687797
Mus musculus
thioredoxin disulfide + prostamide F2alpha
-
-
-
?
thioredoxin + t-butyl hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + ?
-
-
-
?
thioredoxin + tert-butyl hydroperoxide
-
687797
Mus musculus
thioredoxin disulfide + H2O + tert-butanol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 21670, stimulation by ammonium sulfate suggests that the enzyme may be a dimer or oligomer instead of a monomer, calculated from sequence
Mus musculus
?
x * 20000, SDS-PAGE
Sus scrofa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
24
-
assay at
Mus musculus
24
-
assay at
Sus scrofa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.001
-
thioredoxin
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.09
-
(5Z)-N-(2-hydroxyethyl)-7-[(1R,4S,5R,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2,3-dioxabicyclo[2.2.1]hept-5-yl]hept-5-enamide
pH 7.0, 24°C, i.e. prostamide H2
Mus musculus
0.09
-
prostamide H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.09
-
t-butyl hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.09
-
tert-butyl hydroperoxide
pH 7.0, 24°C
Mus musculus
0.1
-
cumene hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C; pH 7.0, 24°C
Mus musculus
0.1
-
H2O2
in 0.1 M KPB (pH 7.0), at 24°C; pH 7.0, 24°C
Mus musculus
0.25
-
(5Z,9alpha,11alpha,13E,15S)-9,11-epidioxy-15-hydroxy-prosta-5,13-dienoate
pH 7.0, 24°C, i.e. prostaglandin H2
Mus musculus
0.25
-
prostaglandin H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
; optimal pH for the prostamide F synthase activity
Mus musculus
7
-
assay at
Sus scrofa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity
Mus musculus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.003
-
H2O2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.22
-
t-butyl hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
3.9
-
cumene hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
11.88
-
prostamide H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
35.85
-
prostaglandin H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.003
-
H2O2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
0.22
-
t-butyl hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
3.9
-
cumene hydroperoxide
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
11.88
-
prostamide H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
35.85
-
prostaglandin H2
in 0.1 M KPB (pH 7.0), at 24°C
Mus musculus
Other publictions for EC 1.11.1.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740108
Urquhart
Endocannabinoids and their oxy ...
Rattus norvegicus
Biochim. Biophys. Acta
1851
366-376
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743717
Iwasa
Prostaglandin F2alpha FP rece ...
Mus musculus
Prostaglandins Leukot. Essent. Fatty Acids
91
175-182
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740711
Silvestri
Anandamide-derived prostamide ...
Mus musculus
J. Biol. Chem.
288
23307-23321
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741103
Woodward
Recent progress in prostagland ...
Mus musculus
Pharmacol. Rev.
65
1135-1147
2013
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707934
Yoshikawa
Preferential localization of p ...
Mus musculus
Brain Res.
1367
22-32
2011
-
-
-
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
9
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
9
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
687797
Moriuchi
Molecular characterization of ...
Mus musculus, Sus scrofa
J. Biol. Chem.
283
792-801
2008
2
-
1
-
3
-
1
12
2
1
3
-
-
5
-
-
2
-
-
18
5
-
15
2
2
-
-
9
2
1
-
1
-
-
-
2
-
1
1
-
3
-
-
1
-
12
2
1
3
-
-
-
-
2
-
18
5
-
15
2
2
-
-
9
2
1
-
-
-
-
-
-
5
5