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Literature summary for 1.11.1.19 extracted from

  • de Eugenio, L.I.; Peces-Perez, R.; Linde, D.; Prieto, A.; Barriuso, J.; Ruiz-Duenas, F.J.; Martinez, M.J.
    Characterization of a dye-decolorizing peroxidase from Irpex lacteus expressed in Escherichia coli An enzyme with wide substrate specificity able to transform lignosulfonates (2021), J. Fungi (Basel), 7, 325 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Irpex lacteus

Inhibitors

Inhibitors Comment Organism Structure
H2O2 the protein becomes progressively inactivated with increasing incubation time and peroxide concentration, detecting the highest inactivation above 100000 molar equivalents. Around 80% inactivation is observed with 3000 molar equivalents in 5 min, and the protein is completely deactivated after 10 min. The activity of the recombinant protein falls below 20% in 60 min of incubation at all the hydrogen peroxide concentrations tested Irpex lacteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) recombinant wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
0.009
-
 Reactive Blue 19 recombinant wild type enzyme, at pH 3.5 and 25°C Irpex lacteus
0.0112
-
 Reactive Black 5 native wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
0.0119
-
 Reactive Black 5 recombinant wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
0.0135
-
 Reactive Blue 19 native wild type enzyme, at pH 3.5 and 25°C Irpex lacteus
0.028
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) native wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
0.0395
-
 H2O2 recombinant wild type enzyme, with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 3.0 and 25°C Irpex lacteus
0.0726
-
 2,6-dimethoxyphenol native wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
0.0795
-
 H2O2 native wild type enzyme, with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 3.0 and 25°C Irpex lacteus
0.082
-
 Mn2+ recombinant wild type enzyme, at pH 4.5 and 25°C Irpex lacteus
0.2938
-
 Mn2+ native wild type enzyme, at pH 4.5 and 25°C Irpex lacteus
0.386
-
 2,6-dimethoxyphenol recombinant wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
3.61
-
 veratryl alcohol native wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
6.88
-
 veratryl alcohol recombinant wild type enzyme, at pH 2.0 and 25°C Irpex lacteus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
resource Q column chromatography Irpex lacteus

Renatured (Commentary)

Renatured (Comment) Organism
using 1.6 mM GSSG, 0.16 M urea, 0.01 mM hemin, Britton-Robinson buffer pH 6.5, at 4°C Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Irpex lacteus oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Irpex lacteus oxidized 2,6-dimethoxyphenol + H2O
-
 ?
lignosulfate + H2O2 + H+
-
 Irpex lacteus oxidized lignosulfate + H2O
-
 ?
Mn2+ + H2O2 + H+
-
 Irpex lacteus Mn3+ + 2 H2O
-
 ?
Reactive Black 5 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 19 + H2O2 + H+
-
 Irpex lacteus oxidized Reactive Blue 19 + H2O
-
 ?
veratryl alcohol + H2O2 + H+
-
 Irpex lacteus veratraldehyde + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Irpex lacteus

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Irpex lacteus
DyP
-
 Irpex lacteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 65 the T50 index is adjusted to 48.3°C, after 10 min incubation between 30 and 65°C Irpex lacteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 Mn2+ recombinant wild type enzyme, at pH 4.5 and 25°C Irpex lacteus
1.8
-
 Mn2+ native wild type enzyme, at pH 4.5 and 25°C Irpex lacteus
2.7
-
 veratryl alcohol native wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
3.9
-
 veratryl alcohol recombinant wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
11.9
-
 Reactive Black 5 native wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
12.9
-
 Reactive Black 5 recombinant wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
49.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) recombinant wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
58.5
-
 Reactive Blue 19 recombinant wild type enzyme, at pH 3.5 and 25°C Irpex lacteus
70.9
-
 2,6-dimethoxyphenol native wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
79.9
-
 Reactive Blue 19 native wild type enzyme, at pH 3.5 and 25°C Irpex lacteus
94.5
-
 2,6-dimethoxyphenol recombinant wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
145.3
-
 H2O2 recombinant wild type enzyme, with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 3.0 and 25°C Irpex lacteus
224
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) native wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
419
-
 H2O2 native wild type enzyme, with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 3.0 and 25°C Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 Reactive Blue 19 and Reactive Black 5 decolorization and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) oxidation are maximal at pH 3.0 Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 the enzyme is very stable between pH 3.0-6.0 at 4°C, keeping 90% activity after 168 incubation. At 25°C, the enzyme retains 90% activity between pH 5.0 and 6.0 and 60% and 20% at pH 3.0 and 4.0, respectively, after 70 h incubation Irpex lacteus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Irpex lacteus

pI Value

Organism Comment pI Value Maximum pI Value
Irpex lacteus isoelectric focusing
-
 4.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.57
-
 veratryl alcohol recombinant wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
0.83
-
 veratryl alcohol native wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
6.1
-
 Mn2+ native wild type enzyme, at pH 4.5 and 25°C Irpex lacteus
14.1
-
 Mn2+ recombinant wild type enzyme, at pH 4.5 and 25°C Irpex lacteus
240
-
 2,6-dimethoxyphenol recombinant wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
970
-
 2,6-dimethoxyphenol native wild type enzyme, at pH 2.0 and 25°C Irpex lacteus
1100
-
 Reactive Black 5 native wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
1100
-
 Reactive Black 5 recombinant wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
3680
-
 H2O2 recombinant wild type enzyme, with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 3.0 and 25°C Irpex lacteus
5300
-
 H2O2 native wild type enzyme, with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as cosubstrate, at pH 3.0 and 25°C Irpex lacteus
5900
-
 Reactive Blue 19 native wild type enzyme, at pH 3.5 and 25°C Irpex lacteus
6500
-
 Reactive Blue 19 recombinant wild type enzyme, at pH 3.5 and 25°C Irpex lacteus
8000
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) native wild type enzyme, at pH 3.0 and 25°C Irpex lacteus
33000
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) recombinant wild type enzyme, at pH 3.0 and 25°C Irpex lacteus