Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
E188K | the mutant shows 3-4fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme | Pseudomonas putida |
E188K/A142V | the mutant shows 2fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme | Pseudomonas putida |
E188K/A142V/H125Y | the mutant shows a 100fold enhanced catalytic efficiency (kcat/Km) for 2,6-dimethoxyphenol and 2fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme | Pseudomonas putida |
E188K/H125R | the mutant shows 6fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme | Pseudomonas putida |
H125R | the mutant shows 3-4fold higher activity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in comparison to the wild type enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.042 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
0.044 | - |
H2O2 | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
0.047 | - |
H2O2 | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
0.058 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
0.06 | - |
2,6-dimethoxyphenol | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida | |
0.06 | - |
H2O2 | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
0.07 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
0.08 | - |
2,6-dimethoxyphenol | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.16 | - |
H2O2 | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
0.2 | - |
H2O2 | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida | |
0.2 | - |
H2O2 | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.22 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida | |
0.22 | - |
H2O2 | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.25 | - |
2,6-dimethoxyphenol | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
0.26 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
0.28 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.5 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
1.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
2.5 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida MET94 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-phenylenediamine + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
1,2-phenylenediamine + H2O2 + H+ | - |
Pseudomonas putida MET94 | ? | - |
? | |
1,4-phenylenediamine + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
1,4-phenylenediamine + H2O2 + H+ | - |
Pseudomonas putida MET94 | ? | - |
? | |
2 1,2-phenylenediamine + H2O2 + H+ | - |
Pseudomonas putida | 4a,10a-dihydrophenazine-2,3-diamine + H2O | - |
? | |
2 2,5-diaminobenzenesulfonic acid + H2O2 + H+ | - |
Pseudomonas putida | 2,7-diaminophenazine-1,6-disulfonic acid + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pseudomonas putida | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pseudomonas putida MET94 | ? | - |
? | |
2,5-diaminobenzenesulfonic acid + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Pseudomonas putida MET94 | ? | - |
? | |
3 1,4-phenylenediamine + H2O2 + H+ | - |
Pseudomonas putida | (3E,6E)-3,6-bis[(4-aminophenyl)imino]cyclohexa-1,4-diene-1,4-diamine + H2O | - |
? | |
3 N1-phenylbenzene-1,4-diamine + H2O2 + H+ | - |
Pseudomonas putida | (3E,6E)-3,6-bis[(4-anilinophenyl)imino]-N1-phenylcyclohexa-1,4-diene-1,4-diamine + H2O | - |
? | |
4-aminodiphenylamine + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
acetosyringone + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Pseudomonas putida MET94 | ? | - |
? | |
lignin + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
methyl syringate + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? | |
syringaldehyde + H2O2 + H+ | - |
Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Pseudomonas putida |
DyP | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.03 | - |
2,6-dimethoxyphenol | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.05 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
0.09 | - |
2,6-dimethoxyphenol | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida | |
1.8 | - |
2,6-dimethoxyphenol | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
3.6 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
4.4 | - |
H2O2 | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
4.4 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
6 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
15 | - |
H2O2 | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
15 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
15 | - |
H2O2 | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
15 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
23 | - |
H2O2 | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
23 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
26 | - |
H2O2 | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
26 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
69 | - |
H2O2 | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
69 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
128 | - |
H2O2 | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida | |
128 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.3 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
H2O2 | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
methyl syringate | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
0.4 | - |
2,6-dimethoxyphenol | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.45 | - |
1,2-phenylenediamine | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
0.6 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
0.7 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
1.1 | - |
1,2-phenylenediamine | mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C | Pseudomonas putida | |
1.2 | - |
guaiacol | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
1.2 | - |
syringaldehyde | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
1.5 | - |
2,6-dimethoxyphenol | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida | |
1.6 | - |
lignin | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
2.5 | - |
1,4-phenylenediamine | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
2.6 | - |
acetosyringone | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
2.9 | - |
2,5-diaminobenzenesulfonic acid | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
3.8 | - |
2,5-diaminobenzenesulfonic acid | mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C | Pseudomonas putida | |
4.1 | - |
lignin | mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C | Pseudomonas putida | |
5 | - |
acetosyringone | mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C | Pseudomonas putida | |
7.2 | - |
2,6-dimethoxyphenol | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
9 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
14 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
14 | - |
methyl syringate | mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C | Pseudomonas putida | |
15 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
17 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
18 | - |
1,4-phenylenediamine | mutant enzyme E188K/A142V/H125Y, at pH 8.0 and 25°C | Pseudomonas putida | |
22 | - |
guaiacol | mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C | Pseudomonas putida | |
33 | - |
syringaldehyde | mutant enzyme E188K/A142V/H125Y, at pH 8.5 and 25°C | Pseudomonas putida | |
52 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
60 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida | |
90 | - |
H2O2 | mutant enzyme A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
90 | - |
H2O2 | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
90 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/A142V, at pH 5.5 and 25°C | Pseudomonas putida | |
110 | - |
2,6-dimethoxyphenol | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
120 | - |
H2O2 | mutant enzyme H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
220 | - |
4-aminodiphenylamine | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
250 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
340 | - |
H2O2 | mutant enzyme E188K/A142V/H125Y, at pH 5.5 and 25°C | Pseudomonas putida | |
350 | - |
H2O2 | mutant enzyme E188K/H125R, at pH 4.3 and 25°C | Pseudomonas putida | |
380 | - |
H2O2 | wild type enzyme, at pH 4.3 and 25°C | Pseudomonas putida | |
420 | - |
4-aminodiphenylamine | mutant enzyme E188K/A142V/H125Y, at pH 5.0 and 25°C | Pseudomonas putida | |
640 | - |
H2O2 | mutant enzyme E188K, at pH 4.3 and 25°C | Pseudomonas putida |