Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain MC1061 | Thermobifida fusca YX |
Protein Variants | Comment | Organism |
---|---|---|
D242A | the mutant shows 0.7% of the Reactive Blue 19-decolorizing activity of the wild type protein | Thermobifida fusca YX |
H338A | the mutant lacks the heme cofactor and shows 3% of the Reactive Blue 19-decolorizing activity of the wild type protein | Thermobifida fusca YX |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
H2O2 | in 25 mM citrate buffer (pH 3.5), at 25°C | Thermobifida fusca YX | |
0.029 | - |
Reactive Blue 19 | in 25 mM citrate buffer (pH 3.5), at 25°C | Thermobifida fusca YX |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Thermobifida fusca YX | 5737 | - |
periplasm | Thermobifida fusca DyP requires the Tat system for periplasmic export | Thermobifida fusca YX | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Thermobifida fusca YX |
46000 | - |
1 * 46000, molecular mass of the unprocessed precursor calculated from amino acid sequence | Thermobifida fusca YX |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca YX | Q47KB1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography | Thermobifida fusca YX |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 + H+ | 4.0% activity compared to Reactive Blue 19 | Thermobifida fusca YX | ? | - |
? | |
3,3'-diaminobenzidine + H2O2 + H+ | weak activity | Thermobifida fusca YX | ? | - |
? | |
guaiacol + H2O2 + H+ | 0.7% activity compared to Reactive Blue 19 | Thermobifida fusca YX | ? | - |
? | |
additional information | the enzyme does not function as a catalase-peroxidase | Thermobifida fusca YX | ? | - |
? | |
o-phenylenediamine + H2O2 + H+ | 0.7%activity compared to Reactive Blue 19 | Thermobifida fusca YX | ? | - |
? | |
Reactive Black 5 + H2O2 + H+ | poor decolorization, 1.4% activity compared to Reactive Blue 19 | Thermobifida fusca YX | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Blue 19 + H2O2 + H+ | 100% activity | Thermobifida fusca YX | oxidized Reactive Blue 19 + H2O | - |
? | |
Reactive Blue 4 + H2O2 + H+ | 29.5% activity compared to Reactive Blue 19 | Thermobifida fusca YX | oxidized Reactive Blue 4 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | 0.2% activity compared to Reactive Blue 19 | Thermobifida fusca YX | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000, molecular mass of the unprocessed precursor calculated from amino acid sequence | Thermobifida fusca YX |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Thermobifida fusca YX |
DyP | - |
Thermobifida fusca YX |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
for Reactive Blue 19-decolorizing activity in 25 mM citrate buffer pH 3.5 | Thermobifida fusca YX |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | after incubation for 2 h at 60°C the enzyme loses 50% of its original activity, while at 30°C and 40°C DyP retains its activity after this period. Upon incubation at 60°C, an almost 2fold increase in activity is observed within 10 min | Thermobifida fusca YX |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
H2O2 | in 25 mM citrate buffer (pH 3.5), at 25°C | Thermobifida fusca YX | |
10 | - |
Reactive Blue 19 | in 25 mM citrate buffer (pH 3.5), at 25°C | Thermobifida fusca YX |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
for Reactive Blue 19-decolorizing activity in 25 mM citrate buffer | Thermobifida fusca YX |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | - |
Thermobifida fusca YX |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the enzyme contains noncovalently bound heme as cofactor | Thermobifida fusca YX |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
333 | - |
H2O2 | in 25 mM citrate buffer (pH 3.5), at 25°C | Thermobifida fusca YX | |
345 | - |
Reactive Blue 19 | in 25 mM citrate buffer (pH 3.5), at 25°C | Thermobifida fusca YX |