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Literature summary for 1.11.1.19 extracted from

  • van Bloois, E.; Torres Pazmino, D.E.; Winter, R.T.; Fraaije, M.W.
    A robust and extracellular heme-containing peroxidase from Thermobifida fusca as prototype of a bacterial peroxidase superfamily (2010), Appl. Microbiol. Biotechnol., 86, 1419-1430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain MC1061 Thermobifida fusca YX

Protein Variants

Protein Variants Comment Organism
D242A the mutant shows 0.7% of the Reactive Blue 19-decolorizing activity of the wild type protein Thermobifida fusca YX
H338A the mutant lacks the heme cofactor and shows 3% of the Reactive Blue 19-decolorizing activity of the wild type protein Thermobifida fusca YX

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
H2O2 in 25 mM citrate buffer (pH 3.5), at 25°C Thermobifida fusca YX
0.029
-
Reactive Blue 19 in 25 mM citrate buffer (pH 3.5), at 25°C Thermobifida fusca YX

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Thermobifida fusca YX 5737
-
periplasm Thermobifida fusca DyP requires the Tat system for periplasmic export Thermobifida fusca YX
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Thermobifida fusca YX
46000
-
1 * 46000, molecular mass of the unprocessed precursor calculated from amino acid sequence Thermobifida fusca YX

Organism

Organism UniProt Comment Textmining
Thermobifida fusca YX Q47KB1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography Thermobifida fusca YX

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2 + H+ 4.0% activity compared to Reactive Blue 19 Thermobifida fusca YX ?
-
?
3,3'-diaminobenzidine + H2O2 + H+ weak activity Thermobifida fusca YX ?
-
?
guaiacol + H2O2 + H+ 0.7% activity compared to Reactive Blue 19 Thermobifida fusca YX ?
-
?
additional information the enzyme does not function as a catalase-peroxidase Thermobifida fusca YX ?
-
?
o-phenylenediamine + H2O2 + H+ 0.7%activity compared to Reactive Blue 19 Thermobifida fusca YX ?
-
?
Reactive Black 5 + H2O2 + H+ poor decolorization, 1.4% activity compared to Reactive Blue 19 Thermobifida fusca YX oxidized Reactive Black 5 + H2O
-
?
Reactive Blue 19 + H2O2 + H+ 100% activity Thermobifida fusca YX oxidized Reactive Blue 19 + H2O
-
?
Reactive Blue 4 + H2O2 + H+ 29.5% activity compared to Reactive Blue 19 Thermobifida fusca YX oxidized Reactive Blue 4 + H2O
-
?
veratryl alcohol + H2O2 + H+ 0.2% activity compared to Reactive Blue 19 Thermobifida fusca YX veratraldehyde + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 46000, molecular mass of the unprocessed precursor calculated from amino acid sequence Thermobifida fusca YX

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
Thermobifida fusca YX
DyP
-
Thermobifida fusca YX

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
for Reactive Blue 19-decolorizing activity in 25 mM citrate buffer pH 3.5 Thermobifida fusca YX

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 after incubation for 2 h at 60°C the enzyme loses 50% of its original activity, while at 30°C and 40°C DyP retains its activity after this period. Upon incubation at 60°C, an almost 2fold increase in activity is observed within 10 min Thermobifida fusca YX

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
H2O2 in 25 mM citrate buffer (pH 3.5), at 25°C Thermobifida fusca YX
10
-
Reactive Blue 19 in 25 mM citrate buffer (pH 3.5), at 25°C Thermobifida fusca YX

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
for Reactive Blue 19-decolorizing activity in 25 mM citrate buffer Thermobifida fusca YX

pH Range

pH Minimum pH Maximum Comment Organism
3 4
-
Thermobifida fusca YX

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains noncovalently bound heme as cofactor Thermobifida fusca YX

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
333
-
H2O2 in 25 mM citrate buffer (pH 3.5), at 25°C Thermobifida fusca YX
345
-
Reactive Blue 19 in 25 mM citrate buffer (pH 3.5), at 25°C Thermobifida fusca YX