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Literature summary for 1.11.1.18 extracted from

  • Kaneko, K.; Washio, K.; Umezawa, T.; Matsuda, F.; Morikawa, M.; Okino, T.
    cDNA cloning and characterization of vanadium-dependent bromoperoxidases from the red alga Laurencia nipponica (2014), Biosci. Biotechnol. Biochem., 78, 1310-1319 .
    View publication on PubMed
Search for more literature for 1.11.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Laurencia nipponica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 H2O2 isoform VBPO2, at pH 6.0 and 25°C Laurencia nipponica
0.026
-
 H2O2 isoform VBPO1, at pH 6.0 and 25°C Laurencia nipponica
0.35
-
 Br- isoform VBPO2, at pH 6.0 and 25°C Laurencia nipponica
0.53
-
 Br- isoform VBPO1, at pH 6.0 and 25°C Laurencia nipponica

Metals/Ions

Metals/Ions Comment Organism Structure
Vanadium dependent on Laurencia nipponica

Organism

Organism UniProt Comment Textmining
Laurencia nipponica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni-NTA column chromatography, and DE52 gel filtration Laurencia nipponica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + (3E,6R,7R)-laurediol
-
 Laurencia nipponica deacetyllaurencin + H2O
-
 ?
Br- + H2O2 + monochlorodimedone
-
 Laurencia nipponica monobromo-monochlorodimedone + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 77000, SDS-PAGE Laurencia nipponica

Synonyms

Synonyms Comment Organism
vanadium-dependent bromoperoxidase
-
 Laurencia nipponica
VBPO1 isoform Laurencia nipponica
VBPO2 isoform Laurencia nipponica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Laurencia nipponica