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Literature summary for 1.11.1.18 extracted from

  • Baharum, H.; Chu, W.C.; Teo, S.S.; Ng, K.Y.; Rahim, R.A.; Ho, C.L.
    Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta) (2013), Phytochemistry, 92, 49-59.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Recombinant enzyme and its mutants have no enzyme activities, unless they are activated by Na3VO4 Gracilaria changii

Protein Variants

Protein Variants Comment Organism
R379F increase in affinity for Br-, enables the mutant to oxidize Cl-, decrease in affinity for I- Gracilaria changii
R379H decrease in affinity for Br- Gracilaria changii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
I- wild-type, pH 7.0, temperature not specified in the publication Gracilaria changii
0.1
-
I- mutant R379H, pH 7.0, temperature not specified in the publication Gracilaria changii
0.22
-
I- mutant R379F, pH 7.0, temperature not specified in the publication Gracilaria changii
0.49
-
Br- mutant R379F, pH 7.0, temperature not specified in the publication Gracilaria changii
0.56
-
H2O2 mutant R379F, pH 7.0, temperature not specified in the publication Gracilaria changii
0.71
-
H2O2 wild-type, pH 7.0, temperature not specified in the publication Gracilaria changii
0.73
-
H2O2 mutant R379H, pH 7.0, temperature not specified in the publication Gracilaria changii
4.69
-
Br- wild-type, pH 7.0, temperature not specified in the publication Gracilaria changii
8.32
-
Br- mutant R379H, pH 7.0, temperature not specified in the publication Gracilaria changii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
x * 84000, SDS-PAGE, recombinant protein with His-tag Gracilaria changii

Organism

Organism UniProt Comment Textmining
Gracilaria changii L7YCT6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + monochlorodimedone
-
Gracilaria changii ? + H2O
-
?
I- + H2O2 + monochlorodimedone
-
Gracilaria changii ? + H2O
-
?
additional information no substrate: chloride Gracilaria changii ?
-
?

Subunits

Subunits Comment Organism
? x * 84000, SDS-PAGE, recombinant protein with His-tag Gracilaria changii

Synonyms

Synonyms Comment Organism
VBPO1
-
Gracilaria changii

pH Range

pH Minimum pH Maximum Comment Organism
7 7.4
-
Gracilaria changii

Expression

Organism Comment Expression
Gracilaria changii transcript abundance is relatively higher in seaweed samples treated with 50 parts per thousand artificial seawater compared to those treated in 10 and 30 parts per thousand artificial seawater up