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Literature summary for 1.11.1.18 extracted from
- Vanadium containing bromoperoxidase--insights into the enzymatic mechanism using X-ray crystallography (2009), J. Inorg. Biochem., 103, 617-621.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Corallina pilulifera |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapour diffusion method, two crystal forms are obtained, one hexagonal form using ammonium phosphate as precipitant (form 1) and a second cubic vanadium bound form (form 2). The best crystals of the cubic form 2 containing vanadate are only obtained with the wild type enzyme. The optimised conditions use an initial protein concentration of 18 mg/ml in 50 mM Tris-H2SO4, 0.4 M KBr, 1 mM Na3VO4, 20% (w/v) polyethylene glycol 6000. For the wild type enzyme crystals grown in form 2, a mother liquor substituting the precipitant with 25% polyethylene glycol 400 and 25% polyethylene glycol 6000 is used | Corallina pilulifera |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R397W | the mutant shows a different behaviour on bromide binding compared to the wild type enzyme, (the bromide is missing the hydrogen-binding interaction that held it in the active site of the wild type enzyme) | Corallina pilulifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corallina pilulifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corallina pilulifera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| vanadium containing bromoperoxidase | - |
Corallina pilulifera |
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Release 2023.1 (January 2023)
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