Application | Comment | Organism |
---|---|---|
agriculture | use of versatile peroxidase in enhancing the digestibility of straws is substantiated through proximate and in vitro digestibility analysis, use of versatile peroxidase in increasing the in vitro degradation of straws for enhancing feed utilization in ruminants. Usage of commonly available crop residues such as paddy straw, finger millet straw, foxtail millet straw, little millet straw, and barnyard millet straw (milled to 1 to 2 cm length and dried at a constant temperature of 70°C) in biodegradation studies | Lentinus squarrosulus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Lentinus squarrosulus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in the heme group | Lentinus squarrosulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | Lentinus squarrosulus | - |
2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | Lentinus squarrosulus TAMI004 | - |
2 Mn(III) + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | Lentinus squarrosulus | - |
oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | Lentinus squarrosulus TAMI004 | - |
oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
additional information | Lentinus squarrosulus | versatile peroxidase oxidizes high molecular weight substrates through catalytic tryptophan at the surface resembling lignin peroxidase and Mn2+ to Mn3+ as in manganese peroxidase | ? | - |
- |
|
additional information | Lentinus squarrosulus TAMI004 | versatile peroxidase oxidizes high molecular weight substrates through catalytic tryptophan at the surface resembling lignin peroxidase and Mn2+ to Mn3+ as in manganese peroxidase | ? | - |
- |
|
Reactive Black 5 + 2 H+ + H2O2 | Lentinus squarrosulus | - |
oxidized Reactive Black 5 + 2 H2O | - |
? | |
Reactive Black 5 + 2 H+ + H2O2 | Lentinus squarrosulus TAMI004 | - |
oxidized Reactive Black 5 + 2 H2O | - |
? | |
veratryl alcohol + H2O2 | Lentinus squarrosulus | - |
verytryl aldehyde + 2 H2O | - |
? | |
veratryl alcohol + H2O2 | Lentinus squarrosulus TAMI004 | - |
verytryl aldehyde + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinus squarrosulus | - |
a wild isolate | - |
Lentinus squarrosulus TAMI004 | - |
a wild isolate | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Lentinus squarrosulus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
manganese oxidizing activity is 12 U/ml in submerged and 131 U/ml in solid-state culture | Lentinus squarrosulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | - |
Lentinus squarrosulus | 2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | - |
Lentinus squarrosulus TAMI004 | 2 Mn(III) + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | - |
Lentinus squarrosulus | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | - |
Lentinus squarrosulus TAMI004 | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
additional information | versatile peroxidase oxidizes high molecular weight substrates through catalytic tryptophan at the surface resembling lignin peroxidase and Mn2+ to Mn3+ as in manganese peroxidase | Lentinus squarrosulus | ? | - |
- |
|
additional information | versatile peroxidase oxidizes high molecular weight substrates through catalytic tryptophan at the surface resembling lignin peroxidase and Mn2+ to Mn3+ as in manganese peroxidase | Lentinus squarrosulus TAMI004 | ? | - |
- |
|
Reactive Black 5 + 2 H+ + H2O2 | - |
Lentinus squarrosulus | oxidized Reactive Black 5 + 2 H2O | - |
? | |
Reactive Black 5 + 2 H+ + H2O2 | - |
Lentinus squarrosulus TAMI004 | oxidized Reactive Black 5 + 2 H2O | - |
? | |
veratryl alcohol + H2O2 | - |
Lentinus squarrosulus | verytryl aldehyde + 2 H2O | - |
? | |
veratryl alcohol + H2O2 | - |
Lentinus squarrosulus TAMI004 | verytryl aldehyde + 2 H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
manganese peroxidase activity assay at, substrate Mn(II) | Lentinus squarrosulus |
4.5 | - |
lignin peroxidase activity assay at, substrate Reactive Black 5 | Lentinus squarrosulus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Lentinus squarrosulus |
General Information | Comment | Organism |
---|---|---|
additional information | versatile peroxidase, an extracellular heme protein of the ligninolytic system, is endowed with polyvalent catalytic sites that render this protein with high redox potential | Lentinus squarrosulus |
physiological function | treatment of crops with Lentinus squarrosulus rich in versatile peroxidase show a decrease in neutral detergent fiber, and acid detergent lignin contents, promptig delignification. Screening of wild isolates for a versatile peroxidase having exceptional efficiency for aromatics and manganese oxidation | Lentinus squarrosulus |