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Literature summary for 1.11.1.16 extracted from

  • Schüttmann, I.; Bouws, H.; Szweda, R.; Suckow, M.; Czermak, P.; Zorn, H.
    Induction, characterization, and heterologous expression of a carotenoid degrading versatile peroxidase from Pleurotus sapidus (2014), J. Mol. Catal. B, 103, 79-84 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Hansenula polymorpha strain RB11 Pleurotus sapidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
beta-carotene at 30°C and pH 4.5 Pleurotus sapidus
0.14
-
syringol at 30°C and pH 4.5 Pleurotus sapidus
0.17
-
veratryl alcohol at 30°C and pH 4.5 Pleurotus sapidus
0.18
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at 30°C and pH 4.5 Pleurotus sapidus

Organism

Organism UniProt Comment Textmining
Pleurotus sapidus Q4QZ27
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE FF' column chromatography and Superdex 200 gel filtration Pleurotus sapidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
with beta-carotene as substrate, at 30°C and pH 4.5 Pleurotus sapidus
1.2
-
with syringol as substrate, at 30°C and pH 4.5 Pleurotus sapidus
1.4
-
with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as substrate, at 30°C and pH 4.5 Pleurotus sapidus
2.3
-
with veratryl alcohol as substrate, at 30°C and pH 4.5 Pleurotus sapidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H+ + H2O2
-
Pleurotus sapidus ?
-
?
beta-carotene + H+ + H2O2
-
Pleurotus sapidus ?
-
?
additional information no activity with Phenol Red and Remazol Brilliant Blue Pleurotus sapidus ?
-
?
syringol + H+ + H2O2
-
Pleurotus sapidus ?
-
?
veratryl alcohol + H+ + H2O2
-
Pleurotus sapidus veratraldehyde + H2O
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Pleurotus sapidus
? x * 34600, calculated from amino acid sequence Pleurotus sapidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Pleurotus sapidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1000
-
beta-carotene at 30°C and pH 4.5 Pleurotus sapidus
1600
-
veratryl alcohol at 30°C and pH 4.5 Pleurotus sapidus
12300
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at 30°C and pH 4.5 Pleurotus sapidus
54500
-
syringol at 30°C and pH 4.5 Pleurotus sapidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Pleurotus sapidus

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5 more than 60% activity between pH 3.0 and 6.5 Pleurotus sapidus

pI Value

Organism Comment pI Value Maximum pI Value
Pleurotus sapidus isoelectric focusing
-
3.6