Cloned (Comment) | Organism |
---|---|
expressed in Hansenula polymorpha strain RB11 | Pleurotus sapidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
beta-carotene | at 30°C and pH 4.5 | Pleurotus sapidus | |
0.14 | - |
syringol | at 30°C and pH 4.5 | Pleurotus sapidus | |
0.17 | - |
veratryl alcohol | at 30°C and pH 4.5 | Pleurotus sapidus | |
0.18 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at 30°C and pH 4.5 | Pleurotus sapidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus sapidus | Q4QZ27 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE FF' column chromatography and Superdex 200 gel filtration | Pleurotus sapidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
with beta-carotene as substrate, at 30°C and pH 4.5 | Pleurotus sapidus |
1.2 | - |
with syringol as substrate, at 30°C and pH 4.5 | Pleurotus sapidus |
1.4 | - |
with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as substrate, at 30°C and pH 4.5 | Pleurotus sapidus |
2.3 | - |
with veratryl alcohol as substrate, at 30°C and pH 4.5 | Pleurotus sapidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H+ + H2O2 | - |
Pleurotus sapidus | ? | - |
? | |
beta-carotene + H+ + H2O2 | - |
Pleurotus sapidus | ? | - |
? | |
additional information | no activity with Phenol Red and Remazol Brilliant Blue | Pleurotus sapidus | ? | - |
? | |
syringol + H+ + H2O2 | - |
Pleurotus sapidus | ? | - |
? | |
veratryl alcohol + H+ + H2O2 | - |
Pleurotus sapidus | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Pleurotus sapidus |
? | x * 34600, calculated from amino acid sequence | Pleurotus sapidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pleurotus sapidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1000 | - |
beta-carotene | at 30°C and pH 4.5 | Pleurotus sapidus | |
1600 | - |
veratryl alcohol | at 30°C and pH 4.5 | Pleurotus sapidus | |
12300 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at 30°C and pH 4.5 | Pleurotus sapidus | |
54500 | - |
syringol | at 30°C and pH 4.5 | Pleurotus sapidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Pleurotus sapidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | more than 60% activity between pH 3.0 and 6.5 | Pleurotus sapidus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pleurotus sapidus | isoelectric focusing | - |
3.6 |