Protein Variants | Comment | Organism |
---|---|---|
E249D | no catalytic activity | Pleurotus ostreatus |
E249Q | no catalytic activity | Pleurotus ostreatus |
Q266F | kinetic properties for H2O2 almost identical to those of wild-type, less than half the RNase A-oxidizing activity of wild-type | Pleurotus ostreatus |
R263D | no catalytic activity | Pleurotus ostreatus |
R263N | kinetic properties for H2O2 almost identical to those of wild-type, additional N-glycosylation | Pleurotus ostreatus |
V166/168L | kinetic properties for H2O2 almost identical to those of wild-type | Pleurotus ostreatus |
W170A | kinetic properties for H2O2 almost identical to those of wild-type, no oxidation of veratryl alcohol, decrease in oxodation of RNase A | Pleurotus ostreatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
H2O2 | mutant V166/168L | Pleurotus ostreatus | |
0.0093 | - |
H2O2 | mutant Q266F | Pleurotus ostreatus | |
0.0098 | - |
H2O2 | mutant R263N | Pleurotus ostreatus | |
0.0099 | - |
H2O2 | mutant W170A | Pleurotus ostreatus | |
0.0103 | - |
H2O2 | wild-type | Pleurotus ostreatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Manganese | Km value 0.022 for wild-type, 0.021 for mutant W170A, 0.022 for mutant R263N, 0.026 for mutant Q266F, 0.027 for mutant V166/168L | Pleurotus ostreatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Poly R-478 + H2O2 | decolorization of the dye | Pleurotus ostreatus | ? | - |
? | |
RNase A + H2O2 | - |
Pleurotus ostreatus | ? | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Pleurotus ostreatus | veratraldehyde + H2O | - |
? |