Protein Variants | Comment | Organism |
---|---|---|
additional information | partially purified lignin peroxidase (LiP) from Pseudomonas fluorescens strain LiP-RL5 is immobilized on graphene oxide functionalized MnFe2O4 nanoparticles (10 nm, synthesized by sol-gel auto-combustion) to fabricate a new hyperactive and thermostable nanobiocatalyst, immobilization method evaluation, overview. Immobilized LiP is quite stable at 50°C with the half-life of 14 h and shows higher tolerance towards various metal ions and solvents than free LiP. Immobilized LiP retains 50% of enzyme activity even after nine consecutive runs. When tested against various textile dyes, the immobilized LiP is effective with higher dye decolourization efficiency (up to 88%) within 1 h of incubation at 30°C | Pseudomonas fluorescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas fluorescens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Pseudomonas fluorescens | |
Ca2+ | - |
Pseudomonas fluorescens | |
Cd2+ | - |
Pseudomonas fluorescens | |
Fe3+ | - |
Pseudomonas fluorescens | |
Hg2+ | - |
Pseudomonas fluorescens | |
K+ | - |
Pseudomonas fluorescens | |
Mg2+ | - |
Pseudomonas fluorescens | |
Mn2+ | - |
Pseudomonas fluorescens | |
Na+ | - |
Pseudomonas fluorescens | |
NH4+ | - |
Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens LiP-RL5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme partially by ammonium sulfate fractionation and dialysis | Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.79 | - |
partially purified enzyme, pH 5.5, 30°C | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 veratryl alcohol + H2O2 | - |
Pseudomonas fluorescens | 2 veratryl aldehyde + 2 H2O | - |
? | |
2 veratryl alcohol + H2O2 | - |
Pseudomonas fluorescens LiP-RL5 | 2 veratryl aldehyde + 2 H2O | - |
? | |
bromophenol blue + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens | ? | - |
? | |
bromophenol blue + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens LiP-RL5 | ? | - |
? | |
coomassie brilliant blue + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens | ? | - |
? | |
coomassie brilliant blue + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens LiP-RL5 | ? | - |
? | |
methylene blue + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens | ? | - |
? | |
methylene blue + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens LiP-RL5 | ? | - |
? | |
nigrosine + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens | ? | - |
? | |
nigrosine + H2O2 | decolorization of a textile dye | Pseudomonas fluorescens LiP-RL5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LIP | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Pseudomonas fluorescens |