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Literature summary for 1.11.1.14 extracted from

  • Yadav, M.; Yadav, P.; Yadav, K.D.
    Purification, characterization, and coal depolymerizing activity of lignin peroxidase from Gloeophyllum sepiarium MTCC-1170 (2009), Biochemistry, 74, 1125-1131.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl complete inhibition at 10 mM Gloeophyllum sepiarium
EDTA complete inhibition at 8 mM Gloeophyllum sepiarium
ethylenediamine complete inhibition at 10 mM Gloeophyllum sepiarium
NaCl complete inhibition at 300 mM Gloeophyllum sepiarium
NaN3 complete inhibition at 10 mM Gloeophyllum sepiarium
tetramethylethylenediamine complete inhibition at 8 mM Gloeophyllum sepiarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
veratryl alcohol in 50 mM sodium tartrate buffer, at pH 2.5, 25°C Gloeophyllum sepiarium
0.076
-
H2O2 in 50 mM sodium tartrate buffer, at pH 2.5, 25°C Gloeophyllum sepiarium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
SDS-PAGE Gloeophyllum sepiarium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 Gloeophyllum sepiarium
-
?
-
?
1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 Gloeophyllum sepiarium MTCC-1170
-
?
-
?

Organism

Organism UniProt Comment Textmining
Gloeophyllum sepiarium
-
-
-
Gloeophyllum sepiarium MTCC-1170
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography Gloeophyllum sepiarium

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
enzyme crude filtrate, pH 2.5, 25°C Gloeophyllum sepiarium
27.9
-
enzyme after 6.2fold purification, pH 2.5, 25°C Gloeophyllum sepiarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2
-
Gloeophyllum sepiarium ?
-
?
1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2
-
Gloeophyllum sepiarium MTCC-1170 ?
-
?
humic acid + H2O2
-
Gloeophyllum sepiarium ?
-
?
humic acid + H2O2
-
Gloeophyllum sepiarium MTCC-1170 ?
-
?
veratryl alcohol + H2O2
-
Gloeophyllum sepiarium 3,4-dimethoxybenzaldehyde + H2O
-
?
veratryl alcohol + H2O2
-
Gloeophyllum sepiarium MTCC-1170 3,4-dimethoxybenzaldehyde + H2O
-
?

Synonyms

Synonyms Comment Organism
LIP
-
Gloeophyllum sepiarium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Gloeophyllum sepiarium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30
-
Gloeophyllum sepiarium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
Gloeophyllum sepiarium

pH Range

pH Minimum pH Maximum Comment Organism
2 3
-
Gloeophyllum sepiarium

Cofactor

Cofactor Comment Organism Structure
heme
-
Gloeophyllum sepiarium

General Information

General Information Comment Organism
physiological function LiP shows lignin degrading and coal depolymerization activity Gloeophyllum sepiarium