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Literature summary for 1.11.1.13 extracted from

  • Kamei, I.; Tomitaka, N.; Motoda, T.; Yamasaki, Y.
    Isozyme selective homologous expression of recombinant manganese peroxidase of salt-tolerant white-rot fungus Phlebia sp. MG-60, and its salt-tolerance and thermostability (2021), J. Microbiol. Biotechnol., 32, 248-255 .
    View publication on PubMed
Search for more literature for 1.11.1.13

Cloned(Commentary)

Cloned (Comment) Organism
gene MGmnp1, DNA and amino acid sequence determination and analysis, recombinant expression of extracellular isozyme MGmnp1 in Phlebia sp. strain MG-60 Phlebia sp. MG-60
gene MGmnp2, DNA and amino acid sequence determination and analysis Phlebia sp. MG-60
gene MGmnp3, DNA and amino acid sequence determination and analysis, recombinant expression of extracellular isozyme MGmnp3 in Phlebia sp. strain MG-60 Phlebia sp. MG-60

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl high concentration of NaCl (over 200 mM to 1200 mM) can enhance the activity of isozyme MGMnP1 Phlebia sp. MG-60

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2 Phlebia sp. MG-60
-
 2 Mn(III) + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Phlebia sp. MG-60 A0A068PC52 MG-60 TUFC40001, isolated from a mangrove forest, three manganese peroxidase (MGMnP) isozymes: MGMnP1, MGMnP2, and MGMnP3
-
Phlebia sp. MG-60 A0A068PCH3 MG-60 TUFC40001, isolated from a mangrove forest, three manganese peroxidase (MGMnP) isozymes: MGMnP1, MGMnP2, and MGMnP3
-
Phlebia sp. MG-60 B1B554 MG-60 TUFC40001, isolated from a mangrove forest, three manganese peroxidase (MGMnP) isozymes: MGMnP1, MGMnP2, and MGMnP3
-

Purification (Commentary)

Purification (Comment) Organism
protoplasts from mycelia, native and recombinant extracellular enzyme from mycelia by filtration, addition of PEG, and anion exchange chromatography (elution with succinate), and ultrafiltration Phlebia sp. MG-60

Source Tissue

Source Tissue Comment Organism Textmining
mycelium this fungus expresses three kinds of manganese peroxidase (MGMnP) isozymes, MGMnP1, MGMnP2, and MGMnP3 in low nitrogen medium (LNM) or LNM containing NaCl, but hardly produces native MnP in a high-nitrogen medium (HNM) Phlebia sp. MG-60
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2
-
 Phlebia sp. MG-60 2 Mn(III) + 2 H2O
-
 ?
2,6-dimethoxyphenol + 2 H+ + H2O2 MnP activity is determined spectrophotometrically by measuring the oxidation of 2,6-dimethoxyphenol to coerulignone (epsilon = 49.6 mM/cm) in 50 mM malonate buffer (pH 4.5) containing 1.0 mM MnSO4, 1.0 mM 2,6-dimethoxyphenol, and 0.2 mM H2O2 at 469 nm, 37°C Phlebia sp. MG-60 coerulignone + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, isozyme MGMnP1, SDS-PAGE Phlebia sp. MG-60
? x * 47000, isozyme MGMnP3, SDS-PAGE Phlebia sp. MG-60
? x * 50000, isozyme MGMnP2, SDS-PAGE Phlebia sp. MG-60
More products of the MnP isozyme genes MGmnp1, MGmnp2, and MGmnp3 are identified by peptide mass fingerprinting analysis as the MnP isozymes at 45, 50, and 47 kDa respectively Phlebia sp. MG-60

Synonyms

Synonyms Comment Organism
MGmn2
-
 Phlebia sp. MG-60
MGmnp1
-
 Phlebia sp. MG-60
MGmnp3
-
 Phlebia sp. MG-60
MnP
-
 Phlebia sp. MG-60

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Phlebia sp. MG-60

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 isozyme MGMnP1 shows relatively high thermotolerance compared with the other isozymes Phlebia sp. MG-60
additional information
-
 isozyme MGMnP3 shows relatively low thermotolerance compared with isozyme MGMnP1 Phlebia sp. MG-60
40 60 stable up to 40°C, while activity decreases above. MGMnP1 keeps half of its activity at 60°C after 1 h Phlebia sp. MG-60
40 60 stable up to 40°C, while activity decreases rapidly above for MGMnP3 after 1 h incubation, inactivation at 50°C Phlebia sp. MG-60
45 50 purified isozyme MGMnP2 is stable up to 45°C while the activity decreases around 50°C and disappears completely at 60°C after 1 h Phlebia sp. MG-60

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Phlebia sp. MG-60

Cofactor

Cofactor Comment Organism Structure
heme
-
 Phlebia sp. MG-60

General Information

General Information Comment Organism
evolution MGMnPs may have evolved to adapt to chloride-rich environments, e.g. mangrove forests Phlebia sp. MG-60