Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Gelatoporia subvermispora |
Protein Variants | Comment | Organism |
---|---|---|
S168W | mutation introduced to confer Vversatile peroxidase-type activity, EC 1.11.1.16, on aromatic substrates and dyes. Variant conserves the high catalytic efficiency of isoform MnP6 oxidizing Mn2+ and gains the ability to oxidize veratryl alcohol as well as reactive black 5 | Gelatoporia subvermispora |
T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G | mutation introduced to confer Vversatile peroxidase-type activity, EC 1.11.1.16, on aromatic substrates and dyes. Variant conserves the high catalytic efficiency of isoform MnP6 oxidizing Mn2+ and gains the ability to oxidize veratryl alcohol as well as reactive black 5 | Gelatoporia subvermispora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
Reactive Black 5 | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
0.0085 | - |
Mn(II) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
0.0087 | - |
Mn(II) | wild-type, 25°C, pH 5.0 | Gelatoporia subvermispora | |
0.011 | - |
Mn(II) | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
0.0126 | - |
Reactive Black 5 | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
0.06 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
2.74 | - |
veratryl alcohol | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
21.6 | - |
veratryl alcohol | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gelatoporia subvermispora | M2REM6 | - |
- |
Gelatoporia subvermispora B | M2REM6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | - |
Gelatoporia subvermispora | 2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | - |
Gelatoporia subvermispora B | 2 Mn(III) + 2 H2O | - |
? | |
2 veratryl alcohol + H2O2 | no substrate of wild-type | Gelatoporia subvermispora | 2 veratraldehyde + H2O | - |
? | |
2 veratryl alcohol + H2O2 | no substrate of wild-type | Gelatoporia subvermispora B | 2 veratraldehyde + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | no substrate of wild-type | Gelatoporia subvermispora | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | no substrate of wild-type | Gelatoporia subvermispora B | ? | - |
? | |
Reactive Black 5 + H2O2 | no substrate of wild-type | Gelatoporia subvermispora | ? + H2O | - |
? | |
Reactive Black 5 + H2O2 | no substrate of wild-type | Gelatoporia subvermispora B | ? + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MnP6 | - |
Gelatoporia subvermispora |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
veratryl alcohol | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
1.4 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
1.69 | - |
veratryl alcohol | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
7 | - |
Reactive Black 5 | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
7.8 | - |
Reactive Black 5 | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
60.5 | - |
Mn(II) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
66.9 | - |
Mn(II) | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
83 | - |
Mn(II) | wild-type, 25°C, pH 5.0 | Gelatoporia subvermispora |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
veratryl alcohol | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
0.197 | - |
veratryl alcohol | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
23 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
619 | - |
Reactive Black 5 | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
1310 | - |
Reactive Black 5 | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
6060 | - |
Mn(II) | mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0 | Gelatoporia subvermispora | |
7110 | - |
Mn(II) | mutant S168W, 25°C, pH 5.0 | Gelatoporia subvermispora | |
9540 | - |
Mn(II) | wild-type, 25°C, pH 5.0 | Gelatoporia subvermispora |