Literature summary for 1.11.1.11 extracted from

Liu, J.X.; Feng, K.; Duan, A.Q.; Li, H.; Yang, Q.Q.; Xu, Z.S.; Xiong, A.S.
Isolation, purification and characterization of an ascorbate peroxidase from celery and overexpression of the AgAPX1 gene enhanced ascorbate content and drought tolerance in Arabidopsis (2019), BMC Plant Biol., 19, 488 .

Search for more literature for 1.11.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
gene AgAPX1, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), recombinant GFP-tagged enzyme expression in onion epidermal cells for analysis of subcellular localization of AgAPX1. Overexpression of AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture. Net photosynthetic rate considerably decreases in the wild-type compared to transgenic lines under drought stress	Apium graveolens

Cloned (Comment)

Organism

gene AgAPX1, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), recombinant GFP-tagged enzyme expression in onion epidermal cells for analysis of subcellular localization of AgAPX1. Overexpression of AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture. Net photosynthetic rate considerably decreases in the wild-type compared to transgenic lines under drought stress

Apium graveolens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Apium graveolens	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	in the heme group	Apium graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 L-ascorbate + H2O2 + 2 H+	Apium graveolens	-	L-ascorbate + L-dehydroascorbate + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Apium graveolens	-	cv. Jinnanshiqin	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21(DE3)	Apium graveolens

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Apium graveolens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-ascorbate + H2O2 + 2 H+	-	Apium graveolens	L-ascorbate + L-dehydroascorbate + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 33160, recombinant enzyme, sequence calculation, x * 34000, recombinant enzyme, SDS-PAGE	Apium graveolens

Synonyms

Synonyms	Comment	Organism
ascorbate peroxidase	-	Apium graveolens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	recombinant enzyme	Apium graveolens

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	85	activity range, profile overview. Inactivation at 90°C, maximum activity at 55°C	Apium graveolens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	recombinant enzyme	Apium graveolens

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	9.5	activity range, profile overview	Apium graveolens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Apium graveolens

General Information

General Information	Comment	Organism
evolution	sequence comparison, phylogenetic analysis and tree	Apium graveolens
physiological function	recombinant overexpression of celery enzyme AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture, physiological changes in Arabidopsis leaves exposed to drought stress, phenotype, overview. The AgAPX1 gene seems to be involved in the response of celery to drought stress. The response of the AgAPX1 gene to adversity may be attributed to the conservation of APX sequences among different species	Apium graveolens