Cloned (Comment) | Organism |
---|---|
into the vector pET-28a+ for the expression in Escherichia coli BL21DE3 cells, the 5'-flanking region of the PgAPX1 gene is cloned into the Topo-TA vector | Cenchrus americanus |
General Stability | Organism |
---|---|
the enzyme is very unstable in the absence of ascorbate and looses its activity within min particularly in the case of chloroplastic and mitochondrial isoforms | Cenchrus americanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | - |
Cenchrus americanus | |
salicylic acid | - |
Cenchrus americanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ascorbate | recombinant PgAPX | Cenchrus americanus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cenchrus americanus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
determined by SDS-PAGE | Cenchrus americanus |
27500 | - |
apparent molecular weight | Cenchrus americanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | Cenchrus americanus | - |
dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cenchrus americanus | A4ZYP9 | - |
- |
Purification (Comment) | Organism |
---|---|
purified near homogeneity from clarified Escherichia coli lysate by passing through Ni-NTA agarose beads | Cenchrus americanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Cenchrus americanus | - |
seedling | - |
Cenchrus americanus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ascorbate + H2O2 | - |
Cenchrus americanus | dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ascorbate peroxidase | - |
Cenchrus americanus |
cytoplasmic ascorbate peroxidase 1 | - |
Cenchrus americanus |
PgAPX1 | - |
Cenchrus americanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
in gel activity assay at room temperature | Cenchrus americanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
in gel activity assay | Cenchrus americanus |
6.6 | - |
activity 75 units mg-1 protein min-1 | Cenchrus americanus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.8 | activity 35 units mg-1 protein min-1 at pH 5.0 and pH 7.8 | Cenchrus americanus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cenchrus americanus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0308 | - |
hydroxylamine | - |
Cenchrus americanus | |
0.1905 | - |
salicylic acid | - |
Cenchrus americanus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cenchrus americanus | estimated isoelectric point | - |
5.62 |
Organism | Comment | Expression |
---|---|---|
Cenchrus americanus | expression of PgAPX1 significantly increases under heat-stress conditions, transcript analysis suggests that the heat-shock element, HSE, motifs may be responsible for stress-specific expression of PgAPX1 by interacting with PgHSF | up |