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Literature summary for 1.11.1.11 extracted from
- Heterologous expression and characterization of a proxidomal ascorbate peroxidase from Populus tomentosa (2007), Mol. Biol. Rep., 36, 21-27.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Populus tomentosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
H2O2 | 25°C | Populus tomentosa |  |
| 0.53 | - |
L-ascorbate | 25°C | Populus tomentosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31580 | - |
2 * 31580, calculated from sequence | Populus tomentosa |
| 37370 | - |
2 * 37370, SDS-PAGE | Populus tomentosa |
| 74000 | - |
gel filtration | Populus tomentosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tomentosa | Q5S1V5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Populus tomentosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Populus tomentosa | - |
| root | - |
Populus tomentosa | - |
| stem | - |
Populus tomentosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-ascorbate + H2O2 | - |
Populus tomentosa | dehydroascorbate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31580, calculated from sequence | Populus tomentosa |
| dimer | 2 * 37370, SDS-PAGE | Populus tomentosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PpAPX | - |
Populus tomentosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Populus tomentosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 55 | 20°C: about 65% of maximal activity, 55°C: about 40% of maximal activity | Populus tomentosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.74 | - |
L-ascorbate | 25°C | Populus tomentosa | |
| 2.03 | - |
H2O2 | 25°C | Populus tomentosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Populus tomentosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | pH 5.0: 68% of maximal activity, pH 9.0: 23% of maximal activity | Populus tomentosa |
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Release 2023.1 (January 2023)
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