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Literature summary for 1.11.1.11 extracted from

  • Kitajima, S.; Kurioka, M.; Yoshimoto, T.; Shindo, M.; Kanaori, K.; Tajima, K.; Oda, K.
    A cysteine residue near the propionate side chain of heme is the radical site in ascorbate peroxidase (2008), FEBS J., 275, 470-480.
    View publication on PubMed
Search for more literature for 1.11.1.11

Protein Variants

Protein Variants Comment Organism
C25S kcat/Km for L-ascorbate is 5.4fold lower than wild-type value. kcat/KM for H2O2 is 2.1fold lower than wild-type value. In contrast to wild-type enzyme, the mutant enzyme retains more than 90% of the initial activity after incubation for 10 min with the radical scavenger 2,2,6,6-tetramethylpiperidinyl-1-oxy and H2O2 Galdieria partita
C25S/C121S kcat/Km for L-ascorbate is 4.5fold lower than wild-type value. kcat/KM for H2O2 is 2.1fold lower than wild-type value. In contrast to wild-type enzyme, the mutant enzyme retains more than 90% of the initial activity after incubation for 10 min with the radical scavenger 2,2,6,6-tetramethylpiperidinyl-1-oxy and H2O2 Galdieria partita
C26S kcat/Km for L-ascorbate is 1.8fold lower than wild-type value. kcat/KM for H2O2 is 3.1fold lower than wild-type value. In contrast to wild-type enzyme, the mutant enzyme retains 60% of the initial activity after incubation for 10 min with the radical scavenger 2,2,6,6-tetramethylpiperidinyl-1-oxy and H2O2 Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
2,2,6,6-tetramethylpiperidinyl-1-oxide formation of 2,2,6,6-tetramethylpiperidinyl-1-oxy-adducts and subsequent oxidation of the cysteine residue located near the propionate group of heme leads to loss of enzyme activity Nicotiana tabacum
2,2,6,6-tetramethylpiperidinyl-1-oxyl radical formation of 2,2,6,6-tetramethylpiperidinyl-1-oxy-adducts and subsequent oxidation of the cysteine residue located near the propionate group of heme leads to loss of enzyme activity Galdieria partita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 H2O2 wild-type enzyme Nicotiana tabacum
0.042
-
 H2O2 wild-type enzyme Galdieria partita
0.05
-
 H2O2 mutant enzyme C25S Galdieria partita
0.052
-
 H2O2 mutant enzyme C25S/C121S Galdieria partita
0.061
-
 H2O2 mutant enzyme C26S Nicotiana tabacum
0.117
-
 L-ascorbate wild-type enzyme Galdieria partita
0.394
-
 L-ascorbate mutant enzyme C25S/C121S Galdieria partita
0.395
-
 L-ascorbate wild-type enzyme Nicotiana tabacum
0.496
-
 L-ascorbate mutant enzyme C25S Galdieria partita
0.566
-
 L-ascorbate mutant enzyme C26S Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma Nicotiana tabacum 9507
-

Organism

Organism UniProt Comment Textmining
Galdieria partita
-
-
-
Nicotiana tabacum Q9TNL9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ascorbate + H2O2
-
 Galdieria partita dehydroascorbate + H2O
-
 ?
L-ascorbate + H2O2
-
 Nicotiana tabacum dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
APX
-
 Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1230
-
 H2O2 mutant enzyme C25S Galdieria partita
1310
-
 H2O2 mutant enzyme C25S/C121S Galdieria partita
1490
-
 L-ascorbate mutant enzyme C25S/C121S Galdieria partita
1570
-
 L-ascorbate mutant enzyme C25S Galdieria partita
1580
-
 H2O2 mutant enzyme C26S Nicotiana tabacum
1800
-
 H2O2 wild-type enzyme Nicotiana tabacum
1980
-
 L-ascorbate wild-type enzyme Galdieria partita
2040
-
 L-ascorbate mutant enzyme C26S Nicotiana tabacum
2190
-
 H2O2 wild-type enzyme Galdieria partita
2510
-
 L-ascorbate wild-type enzyme Nicotiana tabacum