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Literature summary for 1.11.1.11 extracted from

  • Vacca, R.A.; de Pinto, M.C.; Valenti, D.; Passarella, S.; Marra, E.; De Gara, L.
    Production of reactive oxygen species, alteration of cytosolic ascorbate peroxidase, and impairment of mitochondrial metabolism are early events in heat shock-induced programmed cell death in tobacco Bright-Yellow 2 cells (2004), Plant Physiol., 134, 1100-1112.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information untreated cells, sigmoidal dependence of reaction rate on ascorbate concentration, in heat shock cells, hyperbolic dependence of reaction rate on ascorbate concentration and 5-fold decrease in Km-value Nicotiana tabacum
0.038
-
 H2O2 pH 6.4, untreated cells Nicotiana tabacum
0.041
-
 L-ascorbate pH 6.4, heat-shock cells Nicotiana tabacum
0.047
-
 H2O2 pH 6.4, heat-shock cells Nicotiana tabacum
0.229
-
 L-ascorbate pH 6.4, untreated cells Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 Bright-Yellow 2 cells, decrease in enzyme amount and activity upon heat shock leading to programmed cell death
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ascorbate + H2O2
-
 Nicotiana tabacum dehydroascorbate + H2O
-
 ?