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Literature summary for 1.11.1.11 extracted from
- Purification and characterization of ascorbate peroxidase in Chlorella vulgaris (1998), Biochimie, 80, 295-301.
General Stability
| General Stability | Organism |
|---|---|
| the half-inactivation time is estimated to be 15 min, when the enzyme is diluted with ascorbate-depleted medium | Chlorella vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCN | strong inhibition at 1 mM | Chlorella vulgaris |  |
| NaN3 | strong inhibition at 5 mM | Chlorella vulgaris | |
| p-chloromercuribenzoate | 82% inhibition at 0.2 mM for 5 min | Chlorella vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
H2O2 | - |
Chlorella vulgaris | |
| 0.111 | - |
ascorbate | - |
Chlorella vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
gel filtration | Chlorella vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorella vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ultra centrifugation, column chromatography on DEAE-Sephacel, ammonium sulfate precipitation, column chromatography on phenyl Sepharose, ammonium sulfate precipitation and column chromatography on Superdex 200 and Phenyl Superose | Chlorella vulgaris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
cell extract | Chlorella vulgaris |
| 1307 | - |
purified enzyme | Chlorella vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytochrome c + H2O2 | no activity | Chlorella vulgaris | ? + H2O | - |
? | |
| glutathione + H2O2 | no activity | Chlorella vulgaris | ? + H2O | - |
? | |
| guaiacol + H2O2 | no activity | Chlorella vulgaris | ? | - |
? | |
| L-ascorbate + H2O2 | - |
Chlorella vulgaris | dehydroascorbate + H2O | - |
? | |
| L-ascorbic acid + cumene hydroperoxide | no activity | Chlorella vulgaris | dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O | - |
? | |
| L-ascorbic acid + tert-butylhydroperoxide | no activity | Chlorella vulgaris | dehydroascorbate + tert-butylalcohol | - |
? | |
| additional information | no activity with: glutathione, cytochrome c, NADH and NADPH | Chlorella vulgaris | ? | - |
? | |
| NADPH + H2O2 | no activity | Chlorella vulgaris | ? + H2O | - |
? | |
| pyrogallol + H2O2 | 62.6% activity relative to L-ascorbate | Chlorella vulgaris | 3-hydroxybenzo-1,2-quinone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE | Chlorella vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36.4 | - |
- |
Chlorella vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme retains full activity between pH 6 and pH 7.5 | Chlorella vulgaris |
| 60 | - |
complete loss of activity | Chlorella vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 696 | - |
ascorbate | - |
Chlorella vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
- |
Chlorella vulgaris |
| 7 | - |
assay at | Chlorella vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Chlorella vulgaris | |
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