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Literature summary for 1.11.1.10 extracted from

  • Zhang, R.; He, Q.; Huang, Y.; Wang, X.
    Spectroscopic and QM/MM investigations of chloroperoxidase catalyzed degradation of orange G (2016), Arch. Biochem. Biophys., 596, 1-9 .
    View publication on PubMed
Search for more literature for 1.11.1.10

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular docking. Orange G binds within the wide channel of CPO Leptoxyphium fumago

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.131
-
 Orange G presence of Br-, pH 2.7, 20°C Leptoxyphium fumago
0.174
-
 Orange G presence of Cl-, pH 2.7, 20°C Leptoxyphium fumago

Organism

Organism UniProt Comment Textmining
Leptoxyphium fumago
-
-
-
Leptoxyphium fumago ATCC 16373
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leptoxyphium fumago

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azure B + Cl- + H+ + H2O2 70.4% efficiency Leptoxyphium fumago ? + 2 H2O
-
 ?
azure B + Cl- + H+ + H2O2 70.4% efficiency Leptoxyphium fumago ATCC 16373 ? + 2 H2O
-
 ?
gentian violet + Cl- + H+ + H2O2 96.5% efficiency Leptoxyphium fumago ? + 2 H2O
-
 ?
gentian violet + Cl- + H+ + H2O2 96.5% efficiency Leptoxyphium fumago ATCC 16373 ? + 2 H2O
-
 ?
methyl orange + Cl- + H+ + H2O2 98.1% efficiency Leptoxyphium fumago ? + 2 H2O
-
 ?
methyl orange + Cl- + H+ + H2O2 98.1% efficiency Leptoxyphium fumago ATCC 16373 ? + 2 H2O
-
 ?
additional information formation and protonation of compound X, a hypochlorite heme adduct intermediate existing during CPO-catalyzed halide-dependent reactions, significantly lowers the reaction barrier and increases the efficiency of CPO-catalyzed orange G degradation Leptoxyphium fumago ?
-
 ?
additional information formation and protonation of compound X, a hypochlorite heme adduct intermediate existing during CPO-catalyzed halide-dependent reactions, significantly lowers the reaction barrier and increases the efficiency of CPO-catalyzed orange G degradation Leptoxyphium fumago ATCC 16373 ?
-
 ?
nuclear fast red + Cl- + H+ + H2O2 96.7% efficiency Leptoxyphium fumago ? + 2 H2O
-
 ?
nuclear fast red + Cl- + H+ + H2O2 96.7% efficiency Leptoxyphium fumago ATCC 16373 ? + 2 H2O
-
 ?
orange G + Br- + H+ + H2O2
-
 Leptoxyphium fumago ? + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
CPO
-
 Leptoxyphium fumago

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
740
-
 Orange G presence of Br-, pH 2.7, 20°C Leptoxyphium fumago
911
-
 Orange G presence of Cl-, pH 2.7, 20°C Leptoxyphium fumago

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7
-
 the extremely acidic optimal reaction pH suggests the protonation of a residue, presumably, Glu 183 in CPO catalysis Leptoxyphium fumago

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5240
-
 Orange G presence of Cl-, pH 2.7, 20°C Leptoxyphium fumago
5650
-
 Orange G presence of Br-, pH 2.7, 20°C Leptoxyphium fumago