Cloned (Comment) | Organism |
---|---|
expression of E183H in Escherichia coli | Leptoxyphium fumago |
Protein Variants | Comment | Organism |
---|---|---|
E183H | the mutation is detrimental to the chlorination and dismutation activity of chloroperoxidase, activities are reduced by 85% and 50% of the wild-type activity. Epoxidation activity of the mutant enzyme is significantly enhanced, about 2.5fold | Leptoxyphium fumago |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptoxyphium fumago | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Leptoxyphium fumago |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 + methylene blue | - |
Leptoxyphium fumago | oxidized methylene blue + H2O | - |
? | |
monochlorodimedone + Cl- + H2O2 | - |
Leptoxyphium fumago | dichlorodimedone + H2O | - |
? | |
p-nitrostyrene + H2O2 | - |
Leptoxyphium fumago | p-nitrostyrene oxide + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.7 | 5 | wild-type enzyme, chlorination activity | Leptoxyphium fumago |
3 | - |
mutant enzyme E183H, chlorination activity | Leptoxyphium fumago |