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Literature summary for 1.11.1.1 extracted from
- Purification and analysis of streptococcal NADH peroxidase expressed in Escherichia coli (1993), J. Biol. Chem., 268, 3161-3167.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetate | - |
Enterococcus faecalis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Enterococcus faecalis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Enterococcus faecalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | at pH 5.5 in millimolar range | Enterococcus faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values at different pH | Enterococcus faecalis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
4 * 49000, SDS-PAGE | Enterococcus faecalis |
| 201300 | - |
gel filtration | Enterococcus faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
- |
- |
| Enterococcus faecalis 10C1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Enterococcus faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 115 | 125 | - |
Enterococcus faecalis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 49000, SDS-PAGE | Enterococcus faecalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | 47 | fully active after 5 h | Enterococcus faecalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat at different pH values | Enterococcus faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Enterococcus faecalis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
NAD+ | at pH 5.5 | Enterococcus faecalis | |
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