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Literature summary for 1.10.3.6 extracted from

  • Seong, B.L.; Son, H.J.; Mheen, T.I.; Park, Y.H.; Han, M.H.
    Enzymatic oxidation of rifamycins by a microorganism of the genus Humicola (1985), J. Ferment. Technol., 63, 515-522.
No PubMed abstract available
Search for more literature for 1.10.3.6

Application

Application Comment Organism
medicine rifamycin, an ansa-macrolide antibiotic, is used for production of antituberculosic agents Humicola spp.

Inhibitors

Inhibitors Comment Organism Structure
Fe2+
-
 Humicola spp.
Hg2+
-
 Humicola spp.
Rifamycin B concentration above 2 mM Humicola spp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 Rifamycin B
-
 Humicola spp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Humicola spp. 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by other metal ions than Fe2+, Hg2+ Humicola spp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rifamycin B + O2 Humicola spp.
-
 rifamycin O + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Humicola spp.
-
 ATCC 20620
-

Purification (Commentary)

Purification (Comment) Organism
partially, ammonium sulfate precipitation Humicola spp.

Reaction

Reaction Comment Organism Reaction ID
rifamycin B + O2 = rifamycin O + H2O2 acts on various kinds of diphenol compounds, converting them into quinonic forms and H2O2 Humicola spp.
a

Storage Stability

Storage Stability Organism
4°C, 100 mM phosphate buffer, pH 7.8, one month, no appreciable loss of activity Humicola spp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
 Humicola spp. ?
-
 ?
p-hydroquinone + O2
-
 Humicola spp. ?
-
 ?
p-hydroxyphenoxyacetic acid + O2
-
 Humicola spp. ?
-
 ?
rifamycin B + O2
-
 Humicola spp. rifamycin O + H2O2
-
 ?
rifamycin B + O2 most specific, highest catalytic efficiency Humicola spp. rifamycin O + H2O2
-
 ?
rifamycin SV + ?
-
 Humicola spp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 above the catalytic activity is greatly reduced Humicola spp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8
-
 Humicola spp.