Application | Comment | Organism |
---|---|---|
medicine | rifamycin, an ansa-macrolide antibiotic, is used for production of antituberculosic agents | Humicola spp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Humicola spp. | |
Hg2+ | - |
Humicola spp. | |
Rifamycin B | concentration above 2 mM | Humicola spp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Rifamycin B | - |
Humicola spp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Humicola spp. | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by other metal ions than Fe2+, Hg2+ | Humicola spp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rifamycin B + O2 | Humicola spp. | - |
rifamycin O + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humicola spp. | - |
ATCC 20620 | - |
Purification (Comment) | Organism |
---|---|
partially, ammonium sulfate precipitation | Humicola spp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
rifamycin B + O2 = rifamycin O + H2O2 | acts on various kinds of diphenol compounds, converting them into quinonic forms and H2O2 | Humicola spp. |
Storage Stability | Organism |
---|---|
4°C, 100 mM phosphate buffer, pH 7.8, one month, no appreciable loss of activity | Humicola spp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | - |
Humicola spp. | ? | - |
? | |
p-hydroquinone + O2 | - |
Humicola spp. | ? | - |
? | |
p-hydroxyphenoxyacetic acid + O2 | - |
Humicola spp. | ? | - |
? | |
rifamycin B + O2 | - |
Humicola spp. | rifamycin O + H2O2 | - |
? | |
rifamycin B + O2 | most specific, highest catalytic efficiency | Humicola spp. | rifamycin O + H2O2 | - |
? | |
rifamycin SV + ? | - |
Humicola spp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
above the catalytic activity is greatly reduced | Humicola spp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | - |
Humicola spp. |