Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Miyazaki, K.
    A hyperthermophilic laccase from Thermus thermophilus HB27 (2005), Extremophiles, 9, 415-425 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
by signal peptide prediction, the enzyme is assumed to be a secretory protein starting from Gln23. The DNA encoding the mature protein is then cloned and expressed in Escherichia coli BL21 (DE3). The recombinant enzyme, expressed as an apoprotein, is dialyzed against copper-containing buffer to yield a holoprotein Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
EDTA the reaction in the presence of 0.1 mM CuSO4 is completely inhibited by the addition of 1 mM EDTA Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) pH and temperature not specified in the publication Thermus thermophilus
1.88
-
 syringaldazine pH and temperature not specified in the publication Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme requires copper ions for activity. The dependence of the activity on Cu2+ concentration is sigmoidal with the midpoint at 0.00304 mM. Other metal ions (Mg2+, Mn2+, Ca2+, Ni2+, or Zn2+ each at 1 mM) fail to support the activity. The methionine-rich region from Met271 to Met283 may be involved in copper binding Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48986
-
 calculated from sequence Thermus thermophilus
53000
-
 SDS-PAGE, recombinant enzyme Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72HW2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification by signal peptide prediction, the enzyme is assumed to be a secretory protein starting from Gln23 Thermus thermophilus

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Thermus thermophilus ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Thermus thermophilus ?
-
 ?
guaiacol + O2
-
 Thermus thermophilus ?
-
 ?
syringaldazine + O2
-
 Thermus thermophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
TTC1370
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
92
-
 at pH 5.0, maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life: more than 14 h Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.47
-
 syringaldazine pH and temperature not specified in the publication Thermus thermophilus
24.6
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) pH and temperature not specified in the publication Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5 maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine Thermus thermophilus

Expression

Organism Comment Expression
Thermus thermophilus copper-inducible enzyme up