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Literature summary for 1.10.3.2 extracted from

  • Ma, J.; Xu, Z.S.; Wang, F.; Xiong, A.S.
    Isolation, Purification and characterization of two laccases from carrot (Daucus carota L.) and their response to abiotic and metal ions stresses (2015), Protein J., 34, 444-452 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli expression system BL21(DE3) Daucus carota

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Daucus carota
Fe2+
-
 Daucus carota
K+
-
 Daucus carota
Mn2+
-
 Daucus carota
SDS
-
 Daucus carota
Zn2+
-
 Daucus carota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.26
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) laccase DcLac2, pH 2.8, temperature not specified in the publication Daucus carota
3.9
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) laccase DcLac1, pH 2.8, temperature not specified in the publication Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ promotes activity Daucus carota
Cu2+ promotes activity Daucus carota
Mg2+ promotes activity Daucus carota
Na+ promotes activity Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota A0A0U2LZJ6 DcLac2; var. Kuroda
-
Daucus carota A0A0U2US60 DcLac1; var. Kuroda
-

Purification (Commentary)

Purification (Comment) Organism
-
 Daucus carota

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Daucus carota
-
root
-
 Daucus carota
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Daucus carota ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61893, calculated from sequence, DcLac2 Daucus carota
? x * 63991, calculated from sequence, DcLac1 Daucus carota

Synonyms

Synonyms Comment Organism
DcLac1
-
 Daucus carota
DcLac2
-
 Daucus carota

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 laccase DcLac2 Daucus carota
45
-
 laccase DcLac1 Daucus carota

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012988
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) laccase DcLac1, pH 2.8, temperature not specified in the publication Daucus carota
0.018534
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) laccase DcLac2, pH 2.8, temperature not specified in the publication Daucus carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6
-
 laccase DcLac2 Daucus carota
2.8
-
 laccase DcLac1 Daucus carota

Expression

Organism Comment Expression
Daucus carota expression is down-regulated under Mn2+ (0.01 mM), Mg2+ (0.01 mM), Cu2+ (0.01 mM), and K+ (0.01 mM)treatments, besides Cu2+ (0.94-fold), the other three show intensely down-regulated expression down
Daucus carota DcLac1 is upregulated under salt and drought treatments. DcLac1 is strongly upregulated at high and low temperature at early period. The DcLac1 gene is upregulated under Fe2+ (0.01 mM), Zn2+ (0.01 mM) and Ca2+ (0.01 mM) treatments. Zn2+ (0.01 mM) causes 10fold expression of this gene up
Daucus carota DcLac2 is strongly upregulated at high and low temperature at early period. DcLac2 almost shows no extraordinary change under salt and drought treatments. Fe2+ (0.01 mM) causes 4.5-fold upregulation, Cu2+ (0.01 mM) causes 4.8fold upregulation, and K+ (0.01 mM) causes 44.2fold upregulation up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00333
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) laccase DcLac1, pH 2.8, temperature not specified in the publication Daucus carota
0.01477
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) laccase DcLac2, pH 2.8, temperature not specified in the publication Daucus carota