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Literature summary for 1.10.3.2 extracted from

  • Kumar, S.; Jain, K.K.; Rani, S.; Bhardwaj, K.N.; Goel, M.; Kuhad, R.C.
    In-vitro refolding and characterization of recombinant laccase (CotA) from Bacillus pumilus MK001 and its potential for phenolics degradation (2016), Mol. Biotechnol., 58, 789-800 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
efficiently expressed in Escherichia coli BL21(DE3) and process is developed to harvest maximum potential of the enzyme by the refolding of the inclusion bodies Bacillus pumilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1329
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 30°C Bacillus pumilus
7
-
 guaiacol pH 8.0, 30°C Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus I3RYX9
-
-
Bacillus pumilus MK001 I3RYX9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus pumilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus MK001 ?
-
 ?
guaiacol + O2
-
 Bacillus pumilus ?
-
 ?
guaiacol + O2
-
 Bacillus pumilus MK001 ?
-
 ?
additional information a 3D model shows potential binding affinities with ferulic acid, caffeic acid, and vanillin Bacillus pumilus ?
-
 ?
additional information a 3D model shows potential binding affinities with ferulic acid, caffeic acid, and vanillin Bacillus pumilus MK001 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 58880, calculation from sequence Bacillus pumilus

Synonyms

Synonyms Comment Organism
CotA
-
 Bacillus pumilus
spore coat A protein
-
 Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 substrate: guaiacol Bacillus pumilus
80
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 24 h, 10% loss of activity Bacillus pumilus
60
-
 360 min, 40% loss of activity Bacillus pumilus
80
-
 90 min, 25% loss of activity Bacillus pumilus
90
-
 35 min, 30% loss of activity Bacillus pumilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
40
-
 guaiacol pH 8.0, 30°C Bacillus pumilus
377
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 30°C Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus pumilus
8
-
 substrate: guaiacol Bacillus pumilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 24 h, 25% loss of activity Bacillus pumilus
9
-
 24 h, 15% loss of activity Bacillus pumilus
11
-
 24 h, 40% loss of activity Bacillus pumilus

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus pumilus calculation from sequence
-
 5.8