Cloned (Comment) | Organism |
---|---|
expression of a soluble and functional laccase in modified Escherichia coli, trxB2/gor2 mutant(OrigamiTMB (DE3)) at the expression level suitable for industrial application. The choice of Origami is dictated by the fact that disulfide bridges formation is necessary for properfolding of laccase | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 0.1 mM, complete inhibition | Bacillus sp. (in: Bacteria) | |
L-cysteine | 0.1 mM, complete inhibition | Bacillus sp. (in: Bacteria) | |
additional information | no inhibition: EDTA (10-50 mM), SDS (0.1-1 mM) | Bacillus sp. (in: Bacteria) | |
NaCl | 1000 mM, 24% inhibition | Bacillus sp. (in: Bacteria) | |
NaN3 | 1 mM, 35% inhibition | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0196 | - |
syringaldazine | pH 6.5, 37°C, recombinant enzyme | Bacillus sp. (in: Bacteria) | |
0.046 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 30% (v/v), 70% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
dimethyl sulfoxide | 30% (v/v), 65% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Ethanol | 30% (v/v), 65% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Methanol | 30% (v/v), 50% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A0A165W3F6 | - |
- |
Bacillus sp. (in: Bacteria) SL-1 | A0A165W3F6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
recombinant enzyme, substrate: syringaldazine, pH 6.5, 37°C, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) SL-1 | ? | - |
? | |
syringaldazine + O2 | 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
syringaldazine + O2 | 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) SL-1 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 60 min, recombinant enzyme | Bacillus sp. (in: Bacteria) |
80 | - |
half-life: 20 min, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C, recombinant enzyme | Bacillus sp. (in: Bacteria) | |
24 | - |
syringaldazine | pH 6.5, 37°C, recombinant enzyme | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
6.5 | - |
substrate: syringaldazine | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
24 h, complete loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
6.5 | - |
72 h, 22°C, enzyme retains 21% of its activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
8.5 | - |
72 h, 22°C, enzyme retains 17% of its activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |