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Literature summary for 1.10.3.2 extracted from

  • Morales-Alvarez, E.D.; Rivera-Hoyos, C.M.; Cardozo-Bernal, A.M.; Poutou-Pinales, R.A.; Pedroza-Rodriguez, A.M.; Diaz-Rincon, D.J.; Rodriguez-Lopez, A.; Almeciga-Diaz, C.J.; Cuervo-Patino, C.L.
    Plackett-Burman Design for rGILCC1 laccase activity enhancement in Pichia pastoris concentrated enzyme kinetic characterization (2017), Enzyme Res., 2017, 5947581 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Ganoderma lucidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0536
-
 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 25°C, pH 4.5 Ganoderma lucidum

Organism

Organism UniProt Comment Textmining
Ganoderma lucidum V9PQZ7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Ganoderma lucidum ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Ganoderma lucidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, stable up to Ganoderma lucidum
60
-
 1 h, 10% loss of activity Ganoderma lucidum
70
-
 1 h, 40% loss of activity Ganoderma lucidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Ganoderma lucidum

pH Range

pH Minimum pH Maximum Comment Organism
2 11 for pH ranges between pH 2 and pH 11 enzyme activity ranges between 75 and 100% Ganoderma lucidum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 11 1 h, stable Ganoderma lucidum