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Literature summary for 1.10.3.2 extracted from
- Purification and characterization of a new laccase from the filamentous fungus Podospora anserina (2013), Protein Expr. Purif., 88, 61-66.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of a truncated enzyme mutant, lacking the first 31 amino acids, in Pichia pastoris strain GS115 | Podospora anserina |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a truncation mutant lacking the first 31 amino acid residues, the mutant protein displays a better thermostability, with a half life of over 400 min at 37°C, is less sensitive to chloride and more stable at pH 7.0 compared to the wild--type enzyme | Podospora anserina |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0109 | - |
syringaldazine | pH 6.2, 37°C | Podospora anserina |  |
| 0.307 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.2, 37°C | Podospora anserina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | the enzyme contains four copper ions per enzyme molecule | Podospora anserina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62700 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
| 64200 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
| 73000 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
| 79600 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Podospora anserina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris strain GS115 5fold by hydrophobic interaction chromatography | Podospora anserina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 644 | - |
purified recombinant enzyme truncation mutant, pH 3.2, 37°C | Podospora anserina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Podospora anserina | ? | - |
? | |
| syringaldazine + O2 | - |
Podospora anserina | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Podospora anserina |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | activityy range | Podospora anserina |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.29 | - |
syringaldazine | pH 6.2, 37°C | Podospora anserina | |
| 1372 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.2, 37°C | Podospora anserina | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
assay at, with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Podospora anserina |
| 6.2 | - |
assay at, with substrate syringaldazine | Podospora anserina |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Podospora anserina | truncated enzyme mutant, sequence calculation | - |
5.48 |
| Podospora anserina | full-length enzyme, sequence calculation | - |
5.64 |
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