Cloned (Comment) | Organism |
---|---|
overexpression of a truncated enzyme mutant, lacking the first 31 amino acids, in Pichia pastoris strain GS115 | Podospora anserina |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a truncation mutant lacking the first 31 amino acid residues, the mutant protein displays a better thermostability, with a half life of over 400 min at 37°C, is less sensitive to chloride and more stable at pH 7.0 compared to the wild--type enzyme | Podospora anserina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
syringaldazine | pH 6.2, 37°C | Podospora anserina | |
0.307 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.2, 37°C | Podospora anserina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme contains four copper ions per enzyme molecule | Podospora anserina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62700 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
64200 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
73000 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
79600 | - |
x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Podospora anserina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris strain GS115 5fold by hydrophobic interaction chromatography | Podospora anserina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
644 | - |
purified recombinant enzyme truncation mutant, pH 3.2, 37°C | Podospora anserina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Podospora anserina | ? | - |
? | |
syringaldazine + O2 | - |
Podospora anserina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62700, about, full-length enzyme, sequence calculation, x * 64200, about, truncated enzyme mutant, sequence calculation, x * 73000, about, mass spectrometry, x * 79600, SDS-PAGE | Podospora anserina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Podospora anserina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | activityy range | Podospora anserina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.29 | - |
syringaldazine | pH 6.2, 37°C | Podospora anserina | |
1372 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 3.2, 37°C | Podospora anserina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | - |
assay at, with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Podospora anserina |
6.2 | - |
assay at, with substrate syringaldazine | Podospora anserina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Podospora anserina | truncated enzyme mutant, sequence calculation | - |
5.48 |
Podospora anserina | full-length enzyme, sequence calculation | - |
5.64 |