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Literature summary for 1.10.3.2 extracted from
- Kinetic and biochemical properties of high and low redox potential laccases from fungal and plant origin (2010), Biochim. Biophys. Acta, 1804, 899-908.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme expression in Trichoderma reesei under the strong cbh1 (cel7A) promoter in a strain in which the major cellulase gene cbh1 is disrupted | Melanocarpus albomyces |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Trametes versicolor | |
| additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Melanocarpus albomyces | |
| additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Trametes hirsuta | |
| additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Toxicodendron vernicifluum | |
| 0.0024 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Melanocarpus albomyces |  |
| 0.006 | - |
dopamine | pH 5.0, 25°C | Melanocarpus albomyces | |
| 0.031 | - |
catechol | pH 5.0, 25°C | Melanocarpus albomyces | |
| 0.034 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes hirsuta | |
| 0.041 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes hirsuta | |
| 0.045 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.066 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes versicolor | |
| 0.069 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.092 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes versicolor | |
| 0.098 | - |
catechol | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.13 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes versicolor | |
| 0.16 | - |
catechol | pH 5.0, 25°C | Trametes hirsuta | |
| 0.17 | - |
dopamine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.28 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Melanocarpus albomyces | |
| 0.29 | - |
dopamine | pH 5.0, 25°C | Trametes hirsuta | |
| 0.32 | - |
ferrocyanide | pH 5.0, 25°C | Trametes hirsuta | |
| 0.38 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.41 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Melanocarpus albomyces | |
| 0.45 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes hirsuta | |
| 0.79 | - |
ferrocyanide | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.83 | - |
ferrocyanide | pH 5.0, 25°C | Trametes versicolor | |
| 0.89 | - |
ferrocyanide | pH 5.0, 25°C | Melanocarpus albomyces | |
| 1.11 | - |
catechol | pH 5.0, 25°C | Trametes versicolor | |
| 2.1 | - |
dopamine | pH 5.0, 25°C | Trametes versicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, native enzyme, SDS-PAGE | Trametes hirsuta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 catechol + O2 | Trametes versicolor | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | Melanocarpus albomyces | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | Trametes hirsuta | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | Toxicodendron vernicifluum | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | Trametes hirsuta VTT D-95443 | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Melanocarpus albomyces | - |
- |
- |
| Toxicodendron vernicifluum | Q8H979 | - |
- |
| Trametes hirsuta | Q02497 | - |
- |
| Trametes hirsuta VTT D-95443 | Q02497 | - |
- |
| Trametes versicolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from culture filtrate by anion exchange and hydrophobic interaction chromatography | Trametes hirsuta |
| recombinant enzyme from a Trichoderma reeseicbh1 cbh1 disruption mutant strain | Melanocarpus albomyces |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Trametes versicolor | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Trametes versicolor | ? | - |
? | |
| (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
| (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Trametes hirsuta | ? | - |
? | |
| (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
| (2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes versicolor | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes hirsuta | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? | |
| 4 catechol + O2 | - |
Trametes versicolor | 4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | - |
Melanocarpus albomyces | 4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | - |
Trametes hirsuta | 4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | - |
Toxicodendron vernicifluum | 4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4 catechol + O2 | - |
Trametes hirsuta VTT D-95443 | 4 1,2-benzoquinone + 2 H2O | - |
? | |
| 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Trametes versicolor | ? | - |
? | |
| 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Melanocarpus albomyces | ? | - |
? | |
| 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Trametes hirsuta | ? | - |
? | |
| 4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Toxicodendron vernicifluum | ? | - |
? | |
| dopamine + O2 | - |
Trametes versicolor | ? | - |
? | |
| dopamine + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
| dopamine + O2 | - |
Trametes hirsuta | ? | - |
? | |
| dopamine + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
| dopamine + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? | |
| ferrocenemonocarboxylic acid + O2 | - |
Trametes versicolor | ? | - |
? | |
| ferrocenemonocarboxylic acid + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
| ferrocenemonocarboxylic acid + O2 | - |
Trametes hirsuta | ? | - |
? | |
| ferrocenemonocarboxylic acid + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
| ferrocyanide + O2 | - |
Trametes versicolor | ? | - |
? | |
| ferrocyanide + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
| ferrocyanide + O2 | - |
Trametes hirsuta | ? | - |
? | |
| ferrocyanide + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
| ferrocyanide + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, native enzyme, SDS-PAGE | Trametes hirsuta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Trametes versicolor |
| 25 | - |
assay at | Melanocarpus albomyces |
| 25 | - |
assay at | Trametes hirsuta |
| 25 | - |
assay at | Toxicodendron vernicifluum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
ferrocyanide | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.32 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 0.92 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 2.6 | - |
catechol | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 4.4 | - |
dopamine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 10.1 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 64 | - |
ferrocyanide | pH 5.0, 25°C | Melanocarpus albomyces | |
| 75 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Melanocarpus albomyces | |
| 77 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes versicolor | |
| 83 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes versicolor | |
| 88 | - |
catechol | pH 5.0, 25°C | Melanocarpus albomyces | |
| 108 | - |
ferrocyanide | pH 5.0, 25°C | Trametes hirsuta | |
| 109 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Melanocarpus albomyces | |
| 115 | - |
catechol | pH 5.0, 25°C | Trametes versicolor | |
| 116 | - |
dopamine | pH 5.0, 25°C | Melanocarpus albomyces | |
| 122 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes hirsuta | |
| 130 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes versicolor | |
| 137 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes hirsuta | |
| 140 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Melanocarpus albomyces | |
| 184 | - |
dopamine | pH 5.0, 25°C | Trametes hirsuta | |
| 186 | - |
dopamine | pH 5.0, 25°C | Trametes versicolor | |
| 196 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes hirsuta | |
| 202 | - |
catechol | pH 5.0, 25°C | Trametes hirsuta | |
| 215 | - |
ferrocyanide | pH 5.0, 25°C | Trametes versicolor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | assay at | Trametes versicolor |
| 4.5 | 5 | assay at | Melanocarpus albomyces |
| 4.5 | 5 | assay at | Trametes hirsuta |
| 4.5 | 5 | assay at | Toxicodendron vernicifluum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the laccase from Melanocarpus albomyces is a low redox potential enzyme. Structure-function study, mass spectrometry, overview | Melanocarpus albomyces |
| additional information | the laccase from Rhus vernicifera is a low redox potential enzyme. Structure-function study, mass spectrometry, overview | Toxicodendron vernicifluum |
| additional information | the laccase from Trametes hirsuta is a high redox potential enzyme. Structure-function study, mass spectrometry, overview | Trametes hirsuta |
| additional information | the laccase from Trametes versicolor is a high redox potential enzyme. Structure-function study, mass spectrometry, overview | Trametes versicolor |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
ferrocyanide | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 2.3 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 4.3 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 25.1 | - |
dopamine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 26.5 | - |
catechol | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 72 | - |
ferrocyanide | pH 5.0, 25°C | Melanocarpus albomyces | |
| 88 | - |
dopamine | pH 5.0, 25°C | Trametes versicolor | |
| 103 | - |
catechol | pH 5.0, 25°C | Trametes versicolor | |
| 185 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Melanocarpus albomyces | |
| 222 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Toxicodendron vernicifluum | |
| 259 | - |
ferrocyanide | pH 5.0, 25°C | Trametes versicolor | |
| 306 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes hirsuta | |
| 334 | - |
ferrocyanide | pH 5.0, 25°C | Trametes hirsuta | |
| 380 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Melanocarpus albomyces | |
| 643 | - |
dopamine | pH 5.0, 25°C | Trametes hirsuta | |
| 956 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes versicolor | |
| 1024 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes versicolor | |
| 1167 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes versicolor | |
| 1208 | - |
dopamine | pH 5.0, 25°C | Melanocarpus albomyces | |
| 1232 | - |
catechol | pH 5.0, 25°C | Trametes hirsuta | |
| 2838 | - |
catechol | pH 5.0, 25°C | Melanocarpus albomyces | |
| 3288 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes hirsuta | |
| 4780 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes hirsuta | |
| 58330 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Melanocarpus albomyces | |
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