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Literature summary for 1.10.3.2 extracted from

  • Forootanfar, H.; Faramarzi, M.A.; Shahverdi, A.R.; Yazdi, M.T.
    Purification and biochemical characterization of extracellular laccase from the ascomycete Paraconiothyrium variabile (2011), Biores. Technol., 102, 1808-1814.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1% residual activity at 10 mM; 86.6% residual activity at 10 mM Didymosphaeria variabile
Cd2+ 96.2% residual activity at 10 mM Didymosphaeria variabile
dithiothreitol complete inhibition at 0.1 mM Didymosphaeria variabile
EDTA 6.8% residual activity at 1 mM, complete inhibition at 10 mM Didymosphaeria variabile
Fe2+ 8.47% residual activity at 10 mM Didymosphaeria variabile
Hg2+ 7.4% residual activity at 5 mM Didymosphaeria variabile
Mg2+ 84.4% residual activity at 10 mM Didymosphaeria variabile
Mn2+ 2% residual activity at 10 mM Didymosphaeria variabile
additional information 5 mM potassium cyanide shows no inhibitory effect on the laccase Didymosphaeria variabile
Na+ 98% residual activity at 100 mM Didymosphaeria variabile
NaN3 1% residual activity at 10 mM Didymosphaeria variabile
Ni2+ 98.4% residual activity at 10 mM Didymosphaeria variabile
Sodium thioglycolate 45.3% residual activity at 10 mM Didymosphaeria variabile
Zn2+ 75.6% residual activity at 10 mM Didymosphaeria variabile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.203
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in 100 mM citrate buffer, pH 4.5, at 37°C Didymosphaeria variabile
1.114
-
 guaiacol in 100 mM citrate buffer, pH 4.5, at 37°C Didymosphaeria variabile
1.176
-
 2,6-dimethoxyphenol in 100 mM citrate buffer, pH 4.5, at 37°C Didymosphaeria variabile

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 104.7% activity at 10 mM Didymosphaeria variabile
Cu2+ 153.4% activity at 10 mM Didymosphaeria variabile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 SDS-PAGE Didymosphaeria variabile

Organism

Organism UniProt Comment Textmining
Didymosphaeria variabile
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography and Sephadex G-100 gel filtration Didymosphaeria variabile

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Didymosphaeria variabile
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.85
-
 culture filtrate, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Didymosphaeria variabile
18.4
-
 after 21.6fold purification, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Didymosphaeria variabile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Didymosphaeria variabile ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Didymosphaeria variabile ?
-
 ?
guaiacol + O2
-
 Didymosphaeria variabile ?
-
 ?

Synonyms

Synonyms Comment Organism
benzenediol: oxygen oxidoreductase
-
 Didymosphaeria variabile

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Didymosphaeria variabile

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70
-
 Didymosphaeria variabile

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 70 after 24 h incubation at pH 4.8 and 4°C, 80% of the initial activity remains. After 1 h incubation, the enzyme shows about 100% activity at 4°C, about 90% activity at 30°C, about 75% activity at 40°C, about 50% activity at 50°C, about 5% activity at 60°C, and no activity at 70°C Didymosphaeria variabile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Didymosphaeria variabile

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5
-
 Didymosphaeria variabile

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 after 24 h incubation at pH 4.8 and 4°C, 80% of the initial activity remains. After 1 h incubation, the enzyme shows about 65% activity at pH 3.0, about 75% activity at pH 4.0, about 80% activity at pH 5.0, about 60% activity at pH 6.0, about 10% activity at pH 7.0, about 5% activity at pH 8.0, and no activity at pH 9.0 Didymosphaeria variabile

pI Value

Organism Comment pI Value Maximum pI Value
Didymosphaeria variabile isoelectric focusing
-
 4.2