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Literature summary for 1.10.3.2 extracted from

  • Bryjak, J.; Rekuc, A.
    Effective purification of Cerrena unicolor laccase using microfiltration, ultrafiltration and acetone precipitation (2010), Appl. Biochem. Biotechnol., 160, 2219-2235.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Cerrena unicolor
-
-
-
Cerrena unicolor 139
-
-
-

Purification (Commentary)

Purification (Comment) Organism
microfiltration, ultrafiltration and acetone precipitation Cerrena unicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the specific activity of the crude enzyme from culture broth with small amount of brown impurities is 451 units/mg and the specific activity of the 11fold purified enzyme is 5111 units/mg. The specific activity of the crude enzyme from culture broth with big amount of brown impurities is 410 units/mg and the specific activity of the 11.38fold purified enzyme is 4666 units/mg. One unit of proteolytic activity is defined as the amount of enzyme that induces absorbance increase of 1.0 per 10 min at 37°C Cerrena unicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Cerrena unicolor ?
-
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Cerrena unicolor 139 ?
-
?

Synonyms

Synonyms Comment Organism
Benzenediol:oxygen oxidoreductase
-
Cerrena unicolor