Cloned (Comment) | Organism |
---|---|
expression of full-length and amino-terminally truncated laccase-2, both showing lower activity than the native enzyme | Manduca sexta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme shows typical MichaelisMenten kinetics, overview | Manduca sexta | |
0.43 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant N-terminally truncated enzyme | Manduca sexta | |
0.46 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta | |
0.47 | - |
N-beta-alanyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta | |
0.63 | - |
N-acetyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta | |
1.9 | - |
N-beta-alanyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a multicopper oxidase | Manduca sexta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE | Manduca sexta |
90000 | - |
x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE | Manduca sexta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | Q8I8Y0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Manduca sexta |
additional information | laccase-2 is expressed in an active form rather than as a zymogen | Manduca sexta |
Purification (Comment) | Organism |
---|---|
native enzyme from the pharate pupal cuticle to homogeneity | Manduca sexta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | laccase-2 is expressed in an active form rather than as a zymogen | Manduca sexta | - |
pharate pupal cuticle | - |
Manduca sexta | - |
pupal wing cuticle | - |
Manduca sexta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.017 | - |
native enzyme in pharate pupa and pupal wing | Manduca sexta |
0.41 | - |
purified native enzyme, substrate syringaldazine | Manduca sexta |
4.02 | - |
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Manduca sexta |
4.18 | - |
purified native enzyme, substrate N-beta-alanyldopamine-alanyldopamine | Manduca sexta |
5.01 | - |
purified native enzyme, substrate N-acetyldopamine | Manduca sexta |
10.06 | - |
purified native enzyme, substrate hydrochinone | Manduca sexta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Manduca sexta | ? | - |
? | |
hydroquinone + O2 | - |
Manduca sexta | ? | - |
? | |
additional information | no activity with L-tyrosine | Manduca sexta | ? | - |
? | |
N-acetyldopamine + O2 | - |
Manduca sexta | ? | - |
? | |
N-beta-alanyldopamine + O2 | - |
Manduca sexta | ? | - |
? | |
syringaldazine + O2 | - |
Manduca sexta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, full-length enzyme, SDS-PAGE, x * 70000, recombinant N-terminally truncated enzyme, SDS-PAGE | Manduca sexta |
More | peptide mass fingerprinting analysis, mass spectrometry, overview | Manduca sexta |
Synonyms | Comment | Organism |
---|---|---|
laccase-2 | - |
Manduca sexta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.33 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant N-terminally truncated enzyme | Manduca sexta | |
1.67 | - |
N-acetyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta | |
3 | - |
N-beta-alanyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta | |
3.33 | - |
N-beta-alanyldopamine | pH 5.0-5.5, recombinant full-length enzyme | Manduca sexta | |
4.83 | - |
N-acetyldopamine | pH 5.0-5.5, native enzyme | Manduca sexta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Manduca sexta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | pH profiles of native and recombinant enzymes, overview | Manduca sexta |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in cuticle sclerotization | Manduca sexta |