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Literature summary for 1.10.3.2 extracted from
- Purification and characterization of the laccase secreted by the white rot fungus Perenniporia tephropora and its role in the decolourization of synthetic dyes (2007), J. Appl. Microbiol., 102, 1033-1042.
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | the stability of this laccase against metal ions makes the enzyme an efficient agent in the treatment of wastewater containing heavy metals | Perenniporia tephropora |
General Stability
| General Stability | Organism |
|---|---|
| laccase is stable in the presence of some metal ions such as Cu2+, Co2+, Ca2+, Cd2+, Mg2+, Mn2+, Mo2+, Ni2+, Li+ and Al3+ | Perenniporia tephropora |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 100 mM, 92% inhibition | Perenniporia tephropora |  |
| Ca2+ | 100 mM, 43% inhibition | Perenniporia tephropora | |
| Cd2+ | 100 mM, 92% inhibition | Perenniporia tephropora | |
| Co2+ | 100 mM, 51% inhibition | Perenniporia tephropora | |
| EDTA | 0.1 mM, complete inhibition | Perenniporia tephropora | |
| L-cysteine | 5 mM, complete inhibition | Perenniporia tephropora | |
| Mg2+ | 100 mM, 33% inhibition | Perenniporia tephropora | |
| Mn2+ | 100 mM, 44% inhibition | Perenniporia tephropora | |
| Mo2+ | 100 mM, 82% inhibition | Perenniporia tephropora | |
| additional information | no inhibition: SDS, NaBr | Perenniporia tephropora | |
| NaN3 | 0.1 mM, complete inhibition | Perenniporia tephropora | |
| Ni2+ | 100 mM, 52% inhibition | Perenniporia tephropora | |
| p-coumarate | 0.1 mM, complete inhibition | Perenniporia tephropora | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate) | - |
Perenniporia tephropora | |
| 7.3 | - |
2,6-dimethoxyphenol | - |
Perenniporia tephropora | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Perenniporia tephropora | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activates | Perenniporia tephropora | |
| Li+ | activates | Perenniporia tephropora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
1 * 63000, SDS-PAGE | Perenniporia tephropora |
| 65000 | - |
gel filtration | Perenniporia tephropora |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Perenniporia tephropora | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Perenniporia tephropora |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition | malt extract is the carbon source which yields the highest laccase production, with a maximum of laccase production reached after 9 days of cultivation. The nitrogen source soya peptone yields the highest laccase production | Perenniporia tephropora | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 181 | - |
- |
Perenniporia tephropora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| 3,4-dihydroxyphenylacetate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| caffeate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| catechol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| ferulate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| gallate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| guaiacol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| additional information | the crude enzyme as well as the purified laccase is able to decolourize dyes from the textile industries, including remazol brilliant blue R, neolane blue and neolane pink | Perenniporia tephropora | ? | - |
? | |
| protocatechuate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| pyrogallol + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| syringate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| vanillate + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
| vanillin + O2 | - |
Perenniporia tephropora | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 63000, SDS-PAGE | Perenniporia tephropora |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
inactivated rapidly above | Perenniporia tephropora |
| 70 | - |
2 h, complete inactivation | Perenniporia tephropora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
substrate: 2,6-dimethoxyphenol | Perenniporia tephropora |
| 5 | - |
substrate: 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate) | Perenniporia tephropora |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
more stable at alkaline values of pH (8.0) than at acidic values | Perenniporia tephropora |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Perenniporia tephropora | isoelectric focusing | - |
3.3 |
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