KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
syringaldazine | enzyme has a higher affinity for syringaldazine than for L-dopa | Pseudomonas putida | |
0.84 | - |
L-Dopa | enzyme has a higher affinity for syringaldazine than for L-dopa | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
indicated by gel filtration chromatography | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
Pseudomonas putida F6 | - |
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised | - |
Purification (Comment) | Organism |
---|---|
combination of anion exchange chromatography, gel filtration and gel slicing | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
CE, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
0.061 | - |
DEAE, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
0.573 | - |
Resource Q, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
4.57 | - |
gel filtration, purification of Pseudomonas putida F6 laccase-type activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-DOPA + O2 | - |
Pseudomonas putida | ? + H2O | - |
? | |
L-DOPA + O2 | - |
Pseudomonas putida F6 | ? + H2O | - |
? | |
syringaldazine + O2 | - |
Pseudomonas putida | ? | - |
? | |
syringaldazine + O2 | - |
Pseudomonas putida F6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
laccase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable over a broad range of temperatures and are most stable at 30°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, most stable at pH 7.0 | Pseudomonas putida |