Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Yarrowia lipolytica Po1t prototrophic strain | Trametes versicolor |
Protein Variants | Comment | Organism |
---|---|---|
D206A | site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme | Trametes versicolor |
D206E | site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme | Trametes versicolor |
D206N | site-directed mutagenesis, the Asn mutation leads to a significant shift of pH optimum for activity with 2,6-dimethoxyphenol, the mutant shows several fold increased activity compared to the wild-type enzyme | Trametes versicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant mutant D206A | Trametes versicolor | |
0.016 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant mutant D206N | Trametes versicolor | |
0.026 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant mutant D206E | Trametes versicolor | |
0.05 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant wild-type enzyme | Trametes versicolor | |
0.14 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant mutant D206A | Trametes versicolor | |
0.19 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant wild-type enzyme | Trametes versicolor | |
1.63 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant mutant D206N | Trametes versicolor | |
3.28 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant mutant D206E | Trametes versicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | coordination of Cu1 with His458 | Trametes versicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes versicolor | Q96UT7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes versicolor | ? | - |
? | |
2,5-xylidine + O2 | the enzyme also transforms the non-phenolic substrate at pH 5.0, native and recombinant enzymes, wild-type and mutant enzymes, overview | Trametes versicolor | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes versicolor | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
coniferyl alcohol + O2 | the enzyme transforms the non-phenolic substrate at pH 4.5 only when recombinantly expressed in the yeast Yarrowia lipolytica strain Po1t, overview | Trametes versicolor | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Trametes versicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant mutant D206E | Trametes versicolor | |
0.42 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant mutant D206A | Trametes versicolor | |
0.51 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant wild-type enzyme | Trametes versicolor | |
0.66 | - |
2,6-dimethoxyphenol | pH 3.4, 30°C, recombinant mutant D206N | Trametes versicolor | |
1.3 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant wild-type enzyme | Trametes versicolor | |
1.6 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant mutant D206A | Trametes versicolor | |
3.3 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant mutant D206E | Trametes versicolor | |
4.3 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 30°C, recombinant mutant D206N | Trametes versicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 3.4 | assay at, dependent on the substrate | Trametes versicolor |