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Literature summary for 1.10.3.2 extracted from

  • Hakulinen, N.; Kruus, K.; Koivula, A.; Rouvinen, J.
    A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase (2006), Biochem. Biophys. Res. Commun., 350, 929-934.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Trichoderma reesei Melanocarpus albomyces

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, at 22 °C in hanging drops by combining 0.002 ml of 8 mg/ml protein in sodium acetate, pH 5.0, with 0.002 ml of reservoir solution, the reservoir solution contains 13% PMME 2000, 0.1 M ammonium sulfate, and 0.1 M sodium acetate at pH 4.5, microseeding, space group C2, X-ray diffraction structure determination and analysis at 2.0 A resolution Melanocarpus albomyces

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ four coppers involved in dioxygen binding, a copper T1 forming a mononuclear site and a cluster of three coppers T2, T3, and T3' forming a trinuclear site, binding and coordination structure analysis, overview Melanocarpus albomyces

Organism

Organism UniProt Comment Textmining
Melanocarpus albomyces Q70KY3
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Synonyms

Synonyms Comment Organism
blue multicopper oxidase
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Melanocarpus albomyces
p-diphenol dioxygen oxidoreductase
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Melanocarpus albomyces