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Literature summary for 1.10.3.2 extracted from

  • Marques De Souza, C.G.; Peralta, R.M.
    Purification and characterization of the main laccase produced by the white-rot fungus Pleurotus pulmonarius on wheat bran solid state medium (2003), J. Basic Microbiol., 43, 278-286.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol strong Pleurotus pulmonarius
EDTA slight Pleurotus pulmonarius
EGTA slight Pleurotus pulmonarius
Hg2+ strong Pleurotus pulmonarius
Mn2+ slight Pleurotus pulmonarius
Sodium azide strong Pleurotus pulmonarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 syringaldazine pH 6.5, 40°C Pleurotus pulmonarius
0.21
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 40°C Pleurotus pulmonarius
0.55
-
 guaiacol pH 6.5, 40°C Pleurotus pulmonarius

Metals/Ions

Metals/Ions Comment Organism Structure
copper Type I and type II copper center Pleurotus pulmonarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22400
-
 2 * 22400, SDS-PAGE Pleurotus pulmonarius
46000
-
 gel filtration Pleurotus pulmonarius

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone retains high degree of activity Pleurotus pulmonarius
acetonitrile retains high degree of activity Pleurotus pulmonarius

Organism

Organism UniProt Comment Textmining
Pleurotus pulmonarius
-
 main isoform Lcc2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 44% glycosylation Pleurotus pulmonarius

Purification (Commentary)

Purification (Comment) Organism
isoform Lcc2 Pleurotus pulmonarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19750
-
 pH 6.5, 40°C Pleurotus pulmonarius

Storage Stability

Storage Stability Organism
4°C, pH 4.5-8.0, stable for several days Pleurotus pulmonarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Pleurotus pulmonarius ? + H2O
-
 ?
guaiacol + O2
-
 Pleurotus pulmonarius ? + H2O
-
 ?
additional information no substrate: caffeic acid, ferulic acid, tyrosine Pleurotus pulmonarius ?
-
 ?
syringaldazine + O2
-
 Pleurotus pulmonarius ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 22400, SDS-PAGE Pleurotus pulmonarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pleurotus pulmonarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 210 min, 50% residual activity Pleurotus pulmonarius
60
-
 23 min, 50% residual activity Pleurotus pulmonarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
310
-
 guaiacol pH 6.5, 40°C Pleurotus pulmonarius
654
-
 syringaldazine pH 6.5, 40°C Pleurotus pulmonarius
1520
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 40°C Pleurotus pulmonarius

pH Range

pH Minimum pH Maximum Comment Organism
4 5.5 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Pleurotus pulmonarius
6 8 substrate guaiacol Pleurotus pulmonarius
6.2 6.5 substrate syringaldazine Pleurotus pulmonarius

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 8
-
 Pleurotus pulmonarius