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Literature summary for 1.10.3.2 extracted from
- Purification and partial characterization of a thermostable laccase from an unidentified basidiomycete (2004), Enzyme Microb. Technol., 34, 635-641.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | competitive | Basidiomycota |  |
| Sodium azide | strong | Basidiomycota | |
| thioglycolic acid | mixed competitive inhibition after a lag-phase | Basidiomycota | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0123 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.0, 25°C | Basidiomycota | |
| 0.026 | - |
syringic acid | pH 4.5, 25°C | Basidiomycota | |
| 0.251 | - |
guaiacol | pH 6.0, 25°C | Basidiomycota | |
| 6.9 | - |
3-dimethylaminobenzoic acid | pH 6.0, 25°C | Basidiomycota | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Basidiomycota | - |
unidentified fungal isolate | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Basidiomycota | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 52 | - |
pH 4.5, 25°C | Basidiomycota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Basidiomycota | ? + H2O | - |
? | |
| 3-dimethylaminobenzoic acid + 3-methyl-2-benzothiazolinone hydrazone + O2 | ratio of substrates 1:0.07 | Basidiomycota | ? + H2O | - |
? | |
| guaiacol + O2 | - |
Basidiomycota | ? + H2O | - |
? | |
| syringic acid + O2 | - |
Basidiomycota | ? + H2O | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.1 | - |
syringic acid | pH 4.5, 25°C | Basidiomycota | |
| 42.1 | - |
guaiacol | pH 6.0, 25°C | Basidiomycota | |
| 368 | - |
3-dimethylaminobenzoic acid | pH 6.0, 25°C | Basidiomycota | |
| 778 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.0, 25°C | Basidiomycota | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Basidiomycota |
| 4.5 | - |
substrate syringic acid | Basidiomycota |
| 6 | - |
substrates guaiacol, 3-dimethylaminobenzoic acid, 3-methyl-2-benzothiazolinone hydrazone | Basidiomycota |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
Sodium azide | pH 4.5, 25°C | Basidiomycota | |
| 0.802 | - |
thioglycolic acid | pH 4.5, 25°C | Basidiomycota | |
| 1.01 | - |
hydroxylamine | pH 4.5, 25°C | Basidiomycota | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 53 | - |
3-dimethylaminobenzoic acid | pH 6.0, 25°C | Basidiomycota | |
| 168 | - |
guaiacol | pH 6.0, 25°C | Basidiomycota | |
| 388 | - |
syringic acid | pH 4.5, 25°C | Basidiomycota | |
| 63000 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4, 25°C | Basidiomycota | |
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