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Literature summary for 1.10.3.2 extracted from

  • Jung, H.; Xu, F.; Li, K.
    Purification and characterization of laccase from wood-degrading fungus Trichophyton rubrum LKY-7 (2002), Enzyme Microb. Technol., 30, 161-168.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
methyl syringate pH 5.5, 20°C Trichophyton rubrum
0.045
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.5, 20°C Trichophyton rubrum
2.3
-
N-hydroxyacetanilide pH 5.5, 20°C Trichophyton rubrum
58
-
violuric acid pH 5.5, 20°C Trichophyton rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Trichophyton rubrum

Organism

Organism UniProt Comment Textmining
Trichophyton rubrum
-
growth on glucose-peptone medium
-
Trichophyton rubrum LKY-7
-
growth on glucose-peptone medium
-

Purification (Commentary)

Purification (Comment) Organism
purified enzyme has a distinct blue color Trichophyton rubrum

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Trichophyton rubrum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
300
-
pH 5.5, 20°C Trichophyton rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Trichophyton rubrum ? + H2O
-
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Trichophyton rubrum LKY-7 ? + H2O
-
?
2,6-dimethoxyphenol + O2 36% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
?
2,6-dimethoxyphenol + O2 36% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
?
4-methylcatechol + O2 18% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
?
4-methylcatechol + O2 18% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
?
catechol + O2 6.2% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
?
catechol + O2 6.2% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
?
ferulic acid + O2 9.4% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
?
ferulic acid + O2 9.4% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum LKY-7 ? + H2O
-
?
guaiacol + O2 33% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
?
hydroquinone + O2 60% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
?
methyl syringate + O2
-
Trichophyton rubrum ? + H2O
-
?
N-hydroxyacetanilide + O2
-
Trichophyton rubrum ? + H2O
-
?
pyrogallol + O2 2% of activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum ? + H2O
-
?
violuric acid + O2
-
Trichophyton rubrum ? + H2O
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Trichophyton rubrum
More N-terminal amino acid sequence Trichophyton rubrum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
methyl syringate pH 5.5, 20°C Trichophyton rubrum
10
-
violuric acid pH 5.5, 20°C Trichophyton rubrum
10.3
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.5, 20°C Trichophyton rubrum
18.3
-
N-hydroxyacetanilide pH 5.5, 20°C Trichophyton rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Trichophyton rubrum
3.5
-
substrate guaiacol Trichophyton rubrum

pH Range

pH Minimum pH Maximum Comment Organism
3 4
-
Trichophyton rubrum

pI Value

Organism Comment pI Value Maximum pI Value
Trichophyton rubrum isoelectric focusing
-
4