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Literature summary for 1.10.3.2 extracted from

  • Zouari, N.; Romette, J.L.; Thomas, D.
    Purification and properties of two laccase isoenzymes produced by Botrytis cinerea (1987), Appl. Biochem. Biotechnol., 15, 213-225.
No PubMed abstract available
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
azide
-
 Botrytis cinerea
CN-
-
 Botrytis cinerea
diethyldithiocarbamate
-
 Botrytis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.133
-
 O2 laccase 1 Botrytis cinerea
0.533
-
 O2 laccase 2 Botrytis cinerea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72400
-
 x * 72400, SDS-PAGE, probably anomalous migration on SDS-gels Botrytis cinerea
96000 110000 gel filtration, electrophoresis results in 72400 Da Botrytis cinerea

Organism

Organism UniProt Comment Textmining
Botrytis cinerea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sugar content laccase 1: 86%, laccase 2: 91% Botrytis cinerea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.66 28.67 micromol O2/min/mg depending on organism and isozyme Botrytis cinerea

Storage Stability

Storage Stability Organism
-20°C, several months stable Botrytis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,4-benzenetriol + O2
-
 Botrytis cinerea ?
-
 ?
1-naphthol + O2
-
 Botrytis cinerea ? + H2O
-
 ?
2 catechol + O2
-
 Botrytis cinerea 2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
 ?
2-naphthol + O2
-
 Botrytis cinerea ? + H2O
-
 ?
3,5-dimethoxy-hydroxy-benzaldazine + O2
-
 Botrytis cinerea ? + H2O
-
 ?
4,5-dimethyl-o-phenylenediamine + O2
-
 Botrytis cinerea ? + H2O
-
 ?
4-amino-N,N'-dimethylaniline + O2
-
 Botrytis cinerea ? + H2O
-
 ?
4-methylcatechol + O2
-
 Botrytis cinerea 6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
 ?
ascorbate + O2
-
 Botrytis cinerea ?
-
 ?
caffeic acid + O2
-
 Botrytis cinerea ?
-
 ?
gallic acid + O2
-
 Botrytis cinerea ?
-
 ?
guaiacol + O2
-
 Botrytis cinerea 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
additional information overview Botrytis cinerea ?
-
 ?
o-cresol + O2
-
 Botrytis cinerea 6-hydroxy-2-methylcyclohexa-2,4-dienone + H2O
-
 ?
p-cresol + O2
-
 Botrytis cinerea 6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
 ?
p-phenylenediamine + O2
-
 Botrytis cinerea ? + H2O
-
 ?
pyrogallol + O2
-
 Botrytis cinerea ?
-
 ?
quinol + O2
-
 Botrytis cinerea ? + H2O
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 72400, SDS-PAGE, probably anomalous migration on SDS-gels Botrytis cinerea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 laccase 1 Botrytis cinerea
60
-
 laccase 2 Botrytis cinerea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 laccase 2 has higher thermostability than laccase 1 Botrytis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5 3 laccase 1 Botrytis cinerea
4
-
 laccase 2 Botrytis cinerea

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 decline of activity above Botrytis cinerea