BRENDA - Enzyme Database
show all sequences of 1.10.3.12

Characterization of a quinole-oxidase activity in crude extracts of Thermoplasma acidophilum and isolation of an 18-kDa cytochrome

Gartner, P.; Eur. J. Biochem. 200, 215-222 (1991)

Data extracted from this reference:

General Stability
General Stability
Organism
addition of total lipids from Thermoplasma acidophilum increases stability
Thermoplasma acidophilum
asolectin from soya beans decreases stability
Thermoplasma acidophilum
stability is lowered by the presence of detergent
Thermoplasma acidophilum
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-hydroxy-3-undecyl-1,4-naphthoquinone
0.01 mM, 20% inhibition
Thermoplasma acidophilum
2n-heptyl-4-hydroxyquinoline N-oxide
most potent inhibitor
Thermoplasma acidophilum
asolectin
-
Thermoplasma acidophilum
Lipid
addition of total lipids from Thermoplasma acidophilum decreases activity
Thermoplasma acidophilum
additional information
antimycin A, myxothiazol, methyl 3-methoxy-2-(2-styrylphenyl) propenate and stigmatellin (at concentration up to 0.030 mM) and Zn2+ at a concentration up to 1 mM, do not inhibit
Thermoplasma acidophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
ubiquinol-10
pH 5.4, 50°C
Thermoplasma acidophilum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Thermoplasma acidophilum
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thermoplasmaquinol + O2
Thermoplasma acidophilum
likely candidate to the physiological electron donor
thermoplasmaquinone + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermoplasma acidophilum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermoplasma acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,3-dimethylnaphthohydroquinone + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
menadione + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
menaquinol-4 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols
747686
Thermoplasma acidophilum
menaquinone-4 + H2O
-
-
-
?
thermoplasmaquinol + O2
likely candidate to the physiological electron donor
747686
Thermoplasma acidophilum
thermoplasmaquinone + H2O
-
-
-
?
ubiquinol-1 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-1 + H2O
-
-
-
?
ubiquinol-10 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols. However, because of the greater tendency for autoxidation of menaquinols, ubiquinol is a more convenient substrate for the enzyme assay
747686
Thermoplasma acidophilum
ubiquinone-10 + H2O
-
-
-
?
ubiquinol-6 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-6 + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
oligomer
12-16 * 14000, SDS-PAGE
Thermoplasma acidophilum
Synonyms
Synonyms
Commentary
Organism
Q10H2 oxidase
-
Thermoplasma acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
-
Thermoplasma acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome
cytochrome-o-type quinol oxidase
Thermoplasma acidophilum
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00015
-
2n-heptyl-4-hydroxyquinoline N-oxide
pH 5.4, 50°C
Thermoplasma acidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome
cytochrome-o-type quinol oxidase
Thermoplasma acidophilum
General Stability (protein specific)
General Stability
Organism
addition of total lipids from Thermoplasma acidophilum increases stability
Thermoplasma acidophilum
asolectin from soya beans decreases stability
Thermoplasma acidophilum
stability is lowered by the presence of detergent
Thermoplasma acidophilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-hydroxy-3-undecyl-1,4-naphthoquinone
0.01 mM, 20% inhibition
Thermoplasma acidophilum
2n-heptyl-4-hydroxyquinoline N-oxide
most potent inhibitor
Thermoplasma acidophilum
asolectin
-
Thermoplasma acidophilum
Lipid
addition of total lipids from Thermoplasma acidophilum decreases activity
Thermoplasma acidophilum
additional information
antimycin A, myxothiazol, methyl 3-methoxy-2-(2-styrylphenyl) propenate and stigmatellin (at concentration up to 0.030 mM) and Zn2+ at a concentration up to 1 mM, do not inhibit
Thermoplasma acidophilum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.00015
-
2n-heptyl-4-hydroxyquinoline N-oxide
pH 5.4, 50°C
Thermoplasma acidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
ubiquinol-10
pH 5.4, 50°C
Thermoplasma acidophilum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Thermoplasma acidophilum
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thermoplasmaquinol + O2
Thermoplasma acidophilum
likely candidate to the physiological electron donor
thermoplasmaquinone + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,3-dimethylnaphthohydroquinone + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
menadione + O2
-
747686
Thermoplasma acidophilum
?
-
-
-
?
menaquinol-4 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols
747686
Thermoplasma acidophilum
menaquinone-4 + H2O
-
-
-
?
thermoplasmaquinol + O2
likely candidate to the physiological electron donor
747686
Thermoplasma acidophilum
thermoplasmaquinone + H2O
-
-
-
?
ubiquinol-1 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-1 + H2O
-
-
-
?
ubiquinol-10 + O2
menaquinols are oxidized at 6-12fold higher rates than ubiquinols. However, because of the greater tendency for autoxidation of menaquinols, ubiquinol is a more convenient substrate for the enzyme assay
747686
Thermoplasma acidophilum
ubiquinone-10 + H2O
-
-
-
?
ubiquinol-6 + O2
-
747686
Thermoplasma acidophilum
ubiquinone-6 + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
oligomer
12-16 * 14000, SDS-PAGE
Thermoplasma acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
-
Thermoplasma acidophilum
Other publictions for EC 1.10.3.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747686
Gartner
Characterization of a quinole ...
Thermoplasma acidophilum
Eur. J. Biochem.
200
215-222
1991
-
-
-
-
-
3
5
1
1
-
1
1
-
1
-
-
1
-
-
-
-
-
7
1
1
1
-
-
-
1
-
-
1
1
-
-
-
-
-
1
-
-
3
-
5
1
1
1
-
1
1
-
-
-
1
-
-
-
-
7
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-