BRENDA - Enzyme Database show
show all sequences of 1.10.3.11

The cyanide-resistant alternative oxidases from the fungi Pichia stipitis and Neurospora crassa are monomeric and lack regulatory features of the plant enzyme

Umbach, A.L.; Siedow, J.N.; Arch. Biochem. Biophys. 378, 234-245 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ADP
-
Neurospora crassa
ADP
-
Scheffersomyces stipitis
GMP
GMP (0.25 mM) is a strong activator
Neurospora crassa
GMP
GMP (0.25 mM) is a strong activator
Scheffersomyces stipitis
additional information
alternative oxidase activity is not stimulated by pyruvate and glyoxylate. Pyruvate does only stimulate activity when succinate is the respiratory substrate but this is not a direct effect on the alternative oxidase
Neurospora crassa
additional information
alternative oxidase activity is not stimulated by pyruvate and glyoxylate. Pyruvate does only stimulate activity when succinate is the respiratory substrate but this is not a direct effect on the alternative oxidase
Scheffersomyces stipitis
pyruvate
-
Neurospora crassa
pyruvate
-
Scheffersomyces stipitis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Neurospora crassa
5739
-
mitochondrion
-
Scheffersomyces stipitis
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
1 * 36000, SDS-PAGE
Neurospora crassa
36000
-
1 * 36000, SDS-PAGE
Scheffersomyces stipitis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
-
-
-
Scheffersomyces stipitis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
duroquinol + O2
-
713939
Neurospora crassa
duroquinone + H2O
-
-
-
?
duroquinol + O2
-
713939
Scheffersomyces stipitis
duroquinone + H2O
-
-
-
?
additional information
the enzyme is unable to use NADH as respiratory substrate
713939
Neurospora crassa
?
-
-
-
-
additional information
the enzyme is unable to use NADH as respiratory substrate
713939
Scheffersomyces stipitis
?
-
-
-
-
succinate + O2
-
713939
Neurospora crassa
?
-
-
-
?
succinate + O2
-
713939
Scheffersomyces stipitis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Neurospora crassa
monomer
1 * 36000, SDS-PAGE
Scheffersomyces stipitis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ADP
-
Neurospora crassa
ADP
-
Scheffersomyces stipitis
GMP
GMP (0.25 mM) is a strong activator
Neurospora crassa
GMP
GMP (0.25 mM) is a strong activator
Scheffersomyces stipitis
additional information
alternative oxidase activity is not stimulated by pyruvate and glyoxylate. Pyruvate does only stimulate activity when succinate is the respiratory substrate but this is not a direct effect on the alternative oxidase
Neurospora crassa
additional information
alternative oxidase activity is not stimulated by pyruvate and glyoxylate. Pyruvate does only stimulate activity when succinate is the respiratory substrate but this is not a direct effect on the alternative oxidase
Scheffersomyces stipitis
pyruvate
-
Neurospora crassa
pyruvate
-
Scheffersomyces stipitis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Neurospora crassa
5739
-
mitochondrion
-
Scheffersomyces stipitis
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
1 * 36000, SDS-PAGE
Neurospora crassa
36000
-
1 * 36000, SDS-PAGE
Scheffersomyces stipitis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
duroquinol + O2
-
713939
Neurospora crassa
duroquinone + H2O
-
-
-
?
duroquinol + O2
-
713939
Scheffersomyces stipitis
duroquinone + H2O
-
-
-
?
additional information
the enzyme is unable to use NADH as respiratory substrate
713939
Neurospora crassa
?
-
-
-
-
additional information
the enzyme is unable to use NADH as respiratory substrate
713939
Scheffersomyces stipitis
?
-
-
-
-
succinate + O2
-
713939
Neurospora crassa
?
-
-
-
?
succinate + O2
-
713939
Scheffersomyces stipitis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 36000, SDS-PAGE
Neurospora crassa
monomer
1 * 36000, SDS-PAGE
Scheffersomyces stipitis
Other publictions for EC 1.10.3.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
1
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1
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1
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1
1
1
1
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743451
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12-25
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2
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1
1
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-
743543
Sayed
Metabolic interplay between c ...
Symplocarpus renifolius
Plant Signal. Behav.
11
e1247138
2016
-
-
-
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-
-
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5
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1
1
-
-
-
743537
Zhu
Mitochondrial alternative oxi ...
Nicotiana benthamiana
Plant Sci.
239
26-35
2015
-
-
-
-
-
-
-
-
1
-
-
1
-
5
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1
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1
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1
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1
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1
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1
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-
1
1
1
1
-
-
742768
Zhang
-
Characterization of the AOX g ...
Pyropia haitanensis
J. Appl. Phycol.
26
2425-2433
2014
-
-
1
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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-
743464
Tang
Plastid signals induce altern ...
Arabidopsis thaliana
Plant Growth Regul.
74
275-283
2014
-
-
-
-
-
-
-
-
1
-
-
1
-
1
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
2
2
-
-
-
725319
Miura
Cyanide-insensitive quinol oxi ...
Gluconobacter oxydans, Gluconobacter oxydans NBRC 3172
J. Biochem.
153
535-545
2013
-
-
1
-
-
-
6
2
1
2
2
6
-
6
-
-
1
1
-
-
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-
6
1
1
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1
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1
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1
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1
1
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1
6
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2
1
2
2
6
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1
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6
1
1
-
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-
1
-
-
-
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3
3
-
-
-
726398
Shiba
Structure of the trypanosome c ...
Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
110
4580-4585
2013
-
-
-
1
11
-
4
-
-
1
-
1
-
7
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-
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-
2
1
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1
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1
11
-
1
4
-
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1
-
1
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-
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-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726386
Gao
Heme-copper terminal oxidase u ...
Aquifex aeolicus
Proc. Natl. Acad. Sci. USA
109
3275-3280
2012
-
-
-
-
-
-
1
-
1
2
3
1
-
4
-
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1
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2
2
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3
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2
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3
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1
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1
2
3
1
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1
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-
2
2
-
-
-
-
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-
2
-
2
2
-
-
-
713637
Kido
Crystallization and preliminar ...
Trypanosoma brucei brucei, Trypanosoma brucei brucei FN102
Acta Crystallogr. Sect. F
66
275-278
2010
-
-
1
1
-
-
2
-
1
-
2
-
-
4
-
-
1
-
-
-
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2
1
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1
-
1
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2
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1
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2
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1
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2
1
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714308
Yap
The quinone-binding sites of t ...
Escherichia coli
Biochim. Biophys. Acta
1797
1924-1932
2010
-
-
-
-
16
-
3
-
1
1
-
1
-
1
-
-
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-
-
-
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3
-
1
-
-
-
1
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2
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16
-
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3
2
-
1
1
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1
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3
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
714309
Albury
Ubiquinol-binding site in the ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe Sp.011
Biochim. Biophys. Acta
1797
1933-1939
2010
-
-
-
-
7
-
-
-
1
-
2
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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7
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1
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2
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-
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
715509
Shepherd
Compensations for diminished t ...
Escherichia coli, Escherichia coli BW25113
J. Biol. Chem.
285
18464-18472
2010
-
-
-
-
-
-
-
-
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29
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2
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1
1
1
1
-
-
716720
Williams
A broad distribution of the al ...
Antonospora locustae, Trachipleistophora hominis
PLoS Pathog.
6
e1000761
2010
-
-
2
-
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4
-
2
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9
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4
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724413
Crichton
Mutagenesis of the Sauromatum ...
Sauromatum venosum
Biochim. Biophys. Acta
1797
732-737
2010
-
-
1
-
6
-
2
5
2
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1
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5
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1
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2
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715401
Mogi
Biochemical and spectroscopic ...
Gluconobacter oxydans, Gluconobacter oxydans 621H, Gluconobacter oxydans NBRC 3172
J. Biochem.
146
263-271
2009
-
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9
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1
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4
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9
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4
1
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1
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8
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2
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16
18
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2
2
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8
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4
2
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715963
Sootsuwan
Analysis of the respiratory ch ...
Zymomonas mobilis
J. Mol. Microbiol. Biotechnol.
14
163-175
2008
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6
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714299
Umbach
Activation of the plant mitoch ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1554
118-128
2002
1
-
1
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8
-
1
-
2
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1
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4
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1
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2
1
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1
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1
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8
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1
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2
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1
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1
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2
1
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-
715456
Albury
Structure of the plant alterna ...
Sauromatum venosum
J. Biol. Chem.
277
1190-1194
2002
1
-
1
-
4
-
2
-
1
1
-
-
-
5
-
-
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-
-
-
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1
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1
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1
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4
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1
1
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1
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716591
Robson
Transgenic plant cells lacking ...
Nicotiana tabacum
Plant Physiol.
129
1908-1920
2002
-
-
-
-
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3
-
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4
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1
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3
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1
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1
1
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-
713939
Umbach
The cyanide-resistant alternat ...
Neurospora crassa, Scheffersomyces stipitis
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2000
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Siedow
The mitochondrial cyanide-resi ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1459
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2000
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715002
Djajanegara
A single amino acid change in ...
Arabidopsis thaliana, Glycine max
FEBS Lett.
454
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1999
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715441
Affourtit
Functional expression of the p ...
Sauromatum venosum
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1999
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Hoefnagel
Activation of the plant altern ...
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1998
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714291
Berthold
Isolation of mutants of the Ar ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1364
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1998
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716586
Hoefnagel
Substrate kinetics of the plan ...
Arum italicum, Glycine max
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1997
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Albury
Targeting the plant alternativ ...
Sauromatum venosum
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1996
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Millar
Specificity of the organic aci ...
Ipomoea batatas
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714998
Siedow
The active site of the cyanide ...
Sauromatum venosum
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1995
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Moore
Structure-function relationshi ...
Arum maculatum, Sauromatum venosum
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1995
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